Structural organization of pRAM4, a cryptic plasmid from Prevotella ruminicola

Koretsugu Ogata; Rustem I. Aminov; Takafumi Nagamine; Yoshimi Benno; Tsutomu Sekizaki; Makoto Mitsumori; Hajime Minato; Hisao Itabashi

doi:10.1006/plas.1996.0011

Structural organization of pRAM4, a cryptic plasmid from Prevotella ruminicola

Koretsugu Ogata, Rustem I. Aminov^*, Takafumi Nagamine, Yoshimi Benno, Tsutomu Sekizaki, Makoto Mitsumori, Hajime Minato, Hisao Itabashi

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A total of 530 strains of tureen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reading frames, encoding potential polypeptides of M, 32,322 (ORF1) and 32,122 (ORF2) with limited sequence similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequence. The region upstream from ORF1 had an AT-rich (75%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.

Original language	English
Pages (from-to)	91-97
Number of pages	7
Journal	Plasmid
Volume	35
Issue number	2
DOIs	https://doi.org/10.1006/plas.1996.0011
Publication status	Published - Mar 1996

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title = "Structural organization of pRAM4, a cryptic plasmid from Prevotella ruminicola",

abstract = "A total of 530 strains of tureen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reading frames, encoding potential polypeptides of M, 32,322 (ORF1) and 32,122 (ORF2) with limited sequence similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequence. The region upstream from ORF1 had an AT-rich (75%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.",

author = "Koretsugu Ogata and Aminov, {Rustem I.} and Takafumi Nagamine and Yoshimi Benno and Tsutomu Sekizaki and Makoto Mitsumori and Hajime Minato and Hisao Itabashi",

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AU - Ogata, Koretsugu

AU - Aminov, Rustem I.

AU - Nagamine, Takafumi

AU - Benno, Yoshimi

AU - Sekizaki, Tsutomu

AU - Mitsumori, Makoto

AU - Minato, Hajime

AU - Itabashi, Hisao

PY - 1996/3

Y1 - 1996/3

N2 - A total of 530 strains of tureen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reading frames, encoding potential polypeptides of M, 32,322 (ORF1) and 32,122 (ORF2) with limited sequence similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequence. The region upstream from ORF1 had an AT-rich (75%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.

AB - A total of 530 strains of tureen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and completely sequenced. Two open reading frames, encoding potential polypeptides of M, 32,322 (ORF1) and 32,122 (ORF2) with limited sequence similarity to replication initiation and mobilization proteins, respectively, could be identified within the sequence. The region upstream from ORF1 had an AT-rich (75%) region followed by four 22-bp direct repeats, a structure characteristic of replication origins. The plasmid hybridized at high stringency with plasmids from Bacteroides/Prevotella and Butyrivibrio, and with pBR322, suggesting that at least regions of the plasmid are widespread.

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Structural organization of pRAM4, a cryptic plasmid from Prevotella ruminicola

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