The effect of exercise induced cytokines on insulin stimulated glucose transport in C2C12 cells

Stuart Robert Gray; Torkamol Kamolrat

doi:10.1016/j.cyto.2011.04.019

The effect of exercise induced cytokines on insulin stimulated glucose transport in C2C12 cells

Stuart Robert Gray, Torkamol Kamolrat

Medical Sciences

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Abstract

Skeletal muscle contractile activity increases the production of the myokines interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-15 (IL-15) and also skeletal muscle glucose transport. Previous work has revealed a role for IL-6 in mediating glucose uptake, while research on the physiological roles of IL-8 and IL-15 is not so abundant. In the present study we investigated the effects of different concentrations and combinations of IL-6, IL-8 and IL-15 on insulin stimulated glucose transport in C2C12 cells. Furthermore, we also measured AMPK Thr172 and Akt Ser473 phosphorylation via Western blotting. Exposure to 20pg/ml of individual cytokines had no affect on glucose transport while 1ng/ml enhanced (P

Original language	English
Pages (from-to)	221-228
Number of pages	8
Journal	Cytokine
Volume	55
Issue number	2
Early online date	19 May 2011
DOIs	https://doi.org/10.1016/j.cyto.2011.04.019
Publication status	Published - Aug 2011

Keywords

cytokines
glucose uptake
insulin sensitivity

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10.1016/j.cyto.2011.04.019

Cytokine Revision
NOTICE: this is the author’s version of a work that was accepted for publication in Cytokine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cytokine, [VOL 55, ISSUE 2, (2011)] DOI 10.1016/j.cyto.2011.04.019
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AB - Skeletal muscle contractile activity increases the production of the myokines interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-15 (IL-15) and also skeletal muscle glucose transport. Previous work has revealed a role for IL-6 in mediating glucose uptake, while research on the physiological roles of IL-8 and IL-15 is not so abundant. In the present study we investigated the effects of different concentrations and combinations of IL-6, IL-8 and IL-15 on insulin stimulated glucose transport in C2C12 cells. Furthermore, we also measured AMPK Thr172 and Akt Ser473 phosphorylation via Western blotting. Exposure to 20pg/ml of individual cytokines had no affect on glucose transport while 1ng/ml enhanced (P

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The effect of exercise induced cytokines on insulin stimulated glucose transport in C2C12 cells

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Keywords

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