The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses

Susan J. Duthie; Yiannis Mavrommatis; Garry Rucklidge; Martin Reid; Gary Duncan; Mary P. Moyer; Lynn P. Pirie; Charles S. Bestwick

doi:10.1021/pr700751y

The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses

Susan J. Duthie, Yiannis Mavrommatis, Garry Rucklidge, Martin Reid, Gary Duncan, Mary P. Moyer, Lynn P. Pirie, Charles S. Bestwick

INCELL Corp

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].

Original language	English
Pages (from-to)	3254-3266
Number of pages	13
Journal	Journal of Proteome Research
Volume	7
Issue number	8
Early online date	3 Jul 2008
DOIs	https://doi.org/10.1021/pr700751y
Publication status	Published - Aug 2008

Keywords

folic acid deficiency
human colonocytes
NCM460
genomic stability
DNA repair
apoptosis
proteomics
gene-expression profiles
epithelial-cell line
DNA excision-repair
breast-cancer
uracil misincorporation
colon carcinogenesis
colorectal-cancer
mass-spectrometry
carcinoma-cells
protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/pr700751y

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title = "The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses",

abstract = "Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].",

keywords = "folic acid deficiency, human colonocytes, NCM460, genomic stability, DNA repair, apoptosis, proteomics, gene-expression profiles, epithelial-cell line, DNA excision-repair, breast-cancer, uracil misincorporation, colon carcinogenesis, colorectal-cancer, mass-spectrometry, carcinoma-cells, protein",

author = "Duthie, {Susan J.} and Yiannis Mavrommatis and Garry Rucklidge and Martin Reid and Gary Duncan and Moyer, {Mary P.} and Pirie, {Lynn P.} and Bestwick, {Charles S.}",

year = "2008",

month = aug,

doi = "10.1021/pr700751y",

language = "English",

volume = "7",

pages = "3254--3266",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

number = "8",

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TY - JOUR

T1 - The response of human colonocytes to folate deficiency in vitro

T2 - functional and proteomic analyses

AU - Duthie, Susan J.

AU - Mavrommatis, Yiannis

AU - Rucklidge, Garry

AU - Reid, Martin

AU - Duncan, Gary

AU - Moyer, Mary P.

AU - Pirie, Lynn P.

AU - Bestwick, Charles S.

PY - 2008/8

Y1 - 2008/8

N2 - Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].

AB - Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].

KW - folic acid deficiency

KW - human colonocytes

KW - NCM460

KW - genomic stability

KW - DNA repair

KW - apoptosis

KW - proteomics

KW - gene-expression profiles

KW - epithelial-cell line

KW - DNA excision-repair

KW - breast-cancer

KW - uracil misincorporation

KW - colon carcinogenesis

KW - colorectal-cancer

KW - mass-spectrometry

KW - carcinoma-cells

KW - protein

U2 - 10.1021/pr700751y

DO - 10.1021/pr700751y

M3 - Article

SN - 1535-3893

VL - 7

SP - 3254

EP - 3266

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 8

ER -

The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses

Abstract

Keywords

UN SDGs

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