Unhairing wasterwater treatment by Bacillus pumilus and Bacillus cereus.

Najoua Mlaik, Jalel Bouzid, Ines Ben Hassan, Steve Woodward, Lassad Elbahri, Tahar Mechichi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Two proteolytic bacteria Bacillus pumilus and Bacillus cereus were isolated from an aerobic tannery effluent treatment tank. Both strains were characterized and used for the treatment of unhairing wastewater during batch experiments. Chemical oxygen demand (COD), biomass production and protease activity were determined during the incubation period. The
maximum COD removals were of 41 and 44% after 10 days incubation in B. cereus and B. pumilus, respectively. Optimum pH and temperature for protease and keratinase production by both strains were also investigated. Results showed that the optimum
temperature and pH of protease production in the cultures of B. pumilus and B. cereus were of 30˚C and 9.0, respectively. Under these optimum conditions, proteolytic activities were 283.8 and 515.8 U/mL in the cultures of B. cereus and B. pumilus, respectively. Optimum keratinase production by cultures of both bacteria occurred at the same temperature and pH of the protease production.
Original languageEnglish
Pages (from-to)683-689
Number of pages7
JournalDesalination and Water Treatment
Issue number3
Early online date5 Feb 2014
Publication statusPublished - Apr 2015


  • Tannery wastewater
  • Biological treatment
  • Bacillus pumilus
  • Bacillus cereus
  • Protease
  • Keratinase


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