A Bright Future for Fluorescence Imaging of Fungi in Living Hosts

Ambre F. Chapuis; Elizabeth R. Ballou; Donna M. MacCallum

doi:10.3390/jof5020029

A Bright Future for Fluorescence Imaging of Fungi in Living Hosts

Ambre F. Chapuis, Elizabeth R. Ballou, Donna M. MacCallum^* (Corresponding Author)

^*Corresponding author for this work

University of Birmingham

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

11 Downloads (Pure)

Abstract

Traditional in vivo investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have become valuable tools. These new techniques address ethical concerns as they allow reduction in the number of animals required to evaluate new antifungal therapies. They also allow better understanding of the growth and spread of the pathogen during infection. In this review, we concentrate on imaging technologies using different fungal reporter proteins. We discuss the advantages and limitations of these different reporters and compare the efficacy of bioluminescent and fluorescent proteins for fungal research.

Original language	English
Article number	29
Number of pages	9
Journal	Journal of Fungi
Volume	5
Issue number	2
Early online date	3 Apr 2019
DOIs	https://doi.org/10.3390/jof5020029
Publication status	Published - 1 Jun 2019

Bibliographical note

Funding: The PhD studentship (A.C.) was funded by the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), grant number NC/N002482/1. We would also like to acknowledge the support of the Medical Research Council Centre for Medical Mycology at the University of Aberdeen
(MR/N006364/1).

Keywords

flourescent reporters
bioluminescence
iRFP
live imaging
infection models
3Rs
IRFP
Bioluminescence
Infection models
Live imaging
Fluorescent reporters

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10.3390/jof5020029Licence: CC BY

A Bright Future for Fluorescence Imaging of Fungi in Living Hosts
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Final published version, 236 KBLicence: CC BY

Cite this

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abstract = "Traditional in vivo investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have become valuable tools. These new techniques address ethical concerns as they allow reduction in the number of animals required to evaluate new antifungal therapies. They also allow better understanding of the growth and spread of the pathogen during infection. In this review, we concentrate on imaging technologies using different fungal reporter proteins. We discuss the advantages and limitations of these different reporters and compare the efficacy of bioluminescent and fluorescent proteins for fungal research.",

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note = "Funding: The PhD studentship (A.C.) was funded by the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), grant number NC/N002482/1. We would also like to acknowledge the support of the Medical Research Council Centre for Medical Mycology at the University of Aberdeen (MR/N006364/1). ",

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A Bright Future for Fluorescence Imaging of Fungi in Living Hosts

Abstract

Bibliographical note

Keywords

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