TY - JOUR
T1 - A Comparative biochemical study on two marine endophytes bacterium SRCnm and bacillus sp. JS, isolated from red sea algae
AU - Ahmed, Eman Fadl
AU - Hassan, Hossam Mokhtar
AU - Rateb, Mostafa Ezzat
AU - Abdel-Wahab, Noha
AU - Sameer, Somayah
AU - Ebel, Rainer
AU - Taie, Hanan Anwar Aly
AU - Hameed, Mohammed Sayed Abdel
AU - Hammouda, Ola
PY - 2016/1
Y1 - 2016/1
N2 - Two marine endophytic bacteria were isolated from the Red Sea algae; a red alga; Acanthophora dendroides and the brown alga Sargassum sabrepandum. The isolates were identified based on their 16SrRNA sequences as Bacterium SRCnm and Bacillus sp. JS. The objective of this study was to investigate the potential anti-microbial and antioxidant activities of the extracts of the isolated bacteria grown in different nutrient conditions. Compared to amoxicillin (25μ g/disk) and erythromycin (15μ g/disk), the extracts of Bacterium SRCnm in media II, III, IV and V were potent inhibitors of the gram-positive bacterium Sarcina maxima even at low concentrations. Also, the multidrug resistant Staphylococcus aureus (MRSA) was more sensitive to the metabolites produced in medium (II) of the same endophyte than erythromycin (15μ g/disk). A moderate activity of the Bacillus sp. JS extracts of media I and II was obtained against the same pathogen. The total compounds (500ug/ml) of both isolated endophytes showed moderate antioxidant activities (48.9% and 46.1%, respectively). LC/MS analysis of the bacterial extracts was carried out to investigate the likely natural products produced. Cyclo(D-cis-Hyp-L-Leu), dihydrosphingosine and 2-Amino-1,3-hexadecanediol were identified in the fermentation medium of Bacterium SRCnm, whereas cyclo (D-Pro-L-Tyr) and cyclo (L-Leu-L-Pro) were the suggested compounds of Bacillus sp JS.
AB - Two marine endophytic bacteria were isolated from the Red Sea algae; a red alga; Acanthophora dendroides and the brown alga Sargassum sabrepandum. The isolates were identified based on their 16SrRNA sequences as Bacterium SRCnm and Bacillus sp. JS. The objective of this study was to investigate the potential anti-microbial and antioxidant activities of the extracts of the isolated bacteria grown in different nutrient conditions. Compared to amoxicillin (25μ g/disk) and erythromycin (15μ g/disk), the extracts of Bacterium SRCnm in media II, III, IV and V were potent inhibitors of the gram-positive bacterium Sarcina maxima even at low concentrations. Also, the multidrug resistant Staphylococcus aureus (MRSA) was more sensitive to the metabolites produced in medium (II) of the same endophyte than erythromycin (15μ g/disk). A moderate activity of the Bacillus sp. JS extracts of media I and II was obtained against the same pathogen. The total compounds (500ug/ml) of both isolated endophytes showed moderate antioxidant activities (48.9% and 46.1%, respectively). LC/MS analysis of the bacterial extracts was carried out to investigate the likely natural products produced. Cyclo(D-cis-Hyp-L-Leu), dihydrosphingosine and 2-Amino-1,3-hexadecanediol were identified in the fermentation medium of Bacterium SRCnm, whereas cyclo (D-Pro-L-Tyr) and cyclo (L-Leu-L-Pro) were the suggested compounds of Bacillus sp JS.
KW - Antimicrobial activity
KW - Antioxidant
KW - Endophytes
KW - LC/MS analysis
UR - http://www.scopus.com/inward/record.url?scp=84954201712&partnerID=8YFLogxK
M3 - Article
C2 - 26826831
AN - SCOPUS:84954201712
SN - 1011-601X
VL - 29
SP - 17
EP - 26
JO - Pakistan Journal of Pharmaceutical Sciences
JF - Pakistan Journal of Pharmaceutical Sciences
IS - 1
ER -