A failure of meiotic chromosome segregation in a fbh1Δ mutant correlates with persistent Rad51-DNA associations

Weili Sun; Alexander Lorenz; Fekret Osman; Matthew C Whitby

doi:10.1093/nar/gkq977

A failure of meiotic chromosome segregation in a fbh1Δ mutant correlates with persistent Rad51-DNA associations

Weili Sun, Alexander Lorenz, Fekret Osman, Matthew C Whitby

Medical Sciences

University of Oxford

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

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Abstract

The F-box DNA helicase Fbh1 constrains homologous recombination in vegetative cells, most likely through an ability to displace the Rad51 recombinase from DNA. Here, we provide the first evidence that Fbh1 also serves a vital meiotic role in fission yeast to promote normal chromosome segregation. In the absence of Fbh1, chromosomes remain entangled or segregate unevenly during meiosis, and genetic and cytological data suggest that this results in part from a failure to efficiently dismantle Rad51 nucleofilaments that form during meiotic double-strand break repair.

Original language	English
Pages (from-to)	1718-1731
Number of pages	14
Journal	Nucleic Acids Research
Volume	39
Issue number	5
Early online date	11 Dec 2010
DOIs	https://doi.org/10.1093/nar/gkq977
Publication status	Published - Mar 2011

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10.1093/nar/gkq977

gkq977
© The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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abstract = "The F-box DNA helicase Fbh1 constrains homologous recombination in vegetative cells, most likely through an ability to displace the Rad51 recombinase from DNA. Here, we provide the first evidence that Fbh1 also serves a vital meiotic role in fission yeast to promote normal chromosome segregation. In the absence of Fbh1, chromosomes remain entangled or segregate unevenly during meiosis, and genetic and cytological data suggest that this results in part from a failure to efficiently dismantle Rad51 nucleofilaments that form during meiotic double-strand break repair.",

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AU - Osman, Fekret

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A failure of meiotic chromosome segregation in a fbh1Δ mutant correlates with persistent Rad51-DNA associations

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