A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd

Anne P Rehmany; Laura Joy Grenville; Nick D Gunn; Rebecca L. Allen; Zennia Paniwnyk; Jane Byrne; Stephen C Whisson; Paul R. J. Birch; Jim L Beynon

doi:10.1016/S1087-1845(02)00515-7

A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd

Anne P Rehmany, Laura Joy Grenville, Nick D Gunn, Rebecca L. Allen, Zennia Paniwnyk, Jane Byrne, Stephen C Whisson, Paul R. J. Birch, Jim L Beynon

Medical Sciences

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.

Original language	English
Pages (from-to)	33-42
Number of pages	10
Journal	Fungal Genetics and Biology
Volume	38
Issue number	1
Early online date	30 Oct 2002
DOIs	https://doi.org/10.1016/S1087-1845(02)00515-7
Publication status	Published - 1 Feb 2003

Keywords

Alleles
Arabidopsis
Chromosomes, Artificial, Bacterial
Cloning, Molecular
Contig Mapping
DNA
Genetic Markers
Genomic Library
Oomycetes
Physical Chromosome Mapping
Phytophthora

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title = "A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd",

abstract = "In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.",

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AU - Rehmany, Anne P

AU - Grenville, Laura Joy

AU - Gunn, Nick D

AU - Allen, Rebecca L.

AU - Paniwnyk, Zennia

AU - Byrne, Jane

AU - Whisson, Stephen C

AU - Birch, Paul R. J.

AU - Beynon, Jim L

PY - 2003/2/1

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N2 - In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.

AB - In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.

KW - Alleles

KW - Arabidopsis

KW - Chromosomes, Artificial, Bacterial

KW - Cloning, Molecular

KW - Contig Mapping

KW - DNA

KW - Genetic Markers

KW - Genomic Library

KW - Oomycetes

KW - Physical Chromosome Mapping

KW - Phytophthora

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DO - 10.1016/S1087-1845(02)00515-7

M3 - Article

C2 - 12553934

SN - 1087-1845

VL - 38

SP - 33

EP - 42

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

IS - 1

ER -

A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd

Abstract

Keywords

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