A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout

S A  Milne; S  Gallacher; P  Cash; A J R  Porter

doi:10.1016/j.jviromet.2005.09.003

A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout

S A Milne, S Gallacher, P Cash, A J R Porter

Research output: Contribution to journal › Article

19 Citations (Scopus)

Abstract

A new method, termed RT-PCR-ELISA, was evaluated for ease of use, reliability and sensitivity when detecting infectious pancreatic necrosis virus (IPNV) present in trout kidney tissue. The method had comparable sensitivity to existing PCR assays and could successfully detect 1.5 x 10(4) pfu IPNV in artificially contaminated trout kidney samples. The technique was easily established in a new laboratory and required no specialised equipment. The method had a high sample throughput capable of screening 96 samples per run, making the technique extremely time efficient. The RT-PCR-ELISA is a safe, quick, reliable technique, which has the potential for use as a standard virus detection method. (c) 2005 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	92-96
Number of pages	5
Journal	Journal of Virological Methods
Volume	132
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2005.09.003
Publication status	Published - Mar 2006

Keywords

IPNV
detection
RT-PCR
ELISA
reverse-transcriptase PCR
hepatitis-A virus
ennteric viruses
assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jviromet.2005.09.003

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title = "A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout",

abstract = "A new method, termed RT-PCR-ELISA, was evaluated for ease of use, reliability and sensitivity when detecting infectious pancreatic necrosis virus (IPNV) present in trout kidney tissue. The method had comparable sensitivity to existing PCR assays and could successfully detect 1.5 x 10(4) pfu IPNV in artificially contaminated trout kidney samples. The technique was easily established in a new laboratory and required no specialised equipment. The method had a high sample throughput capable of screening 96 samples per run, making the technique extremely time efficient. The RT-PCR-ELISA is a safe, quick, reliable technique, which has the potential for use as a standard virus detection method. (c) 2005 Elsevier B.V. All rights reserved.",

keywords = "IPNV, detection, RT-PCR, ELISA, reverse-transcriptase PCR, hepatitis-A virus, ennteric viruses, assay",

author = "Milne, {S A} and S Gallacher and P Cash and Porter, {A J R}",

year = "2006",

month = mar,

doi = "10.1016/j.jviromet.2005.09.003",

language = "English",

volume = "132",

pages = "92--96",

journal = "Journal of Virological Methods",

publisher = "Elsevier",

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T1 - A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout

AU - Milne, S A

AU - Gallacher, S

AU - Cash, P

AU - Porter, A J R

PY - 2006/3

Y1 - 2006/3

N2 - A new method, termed RT-PCR-ELISA, was evaluated for ease of use, reliability and sensitivity when detecting infectious pancreatic necrosis virus (IPNV) present in trout kidney tissue. The method had comparable sensitivity to existing PCR assays and could successfully detect 1.5 x 10(4) pfu IPNV in artificially contaminated trout kidney samples. The technique was easily established in a new laboratory and required no specialised equipment. The method had a high sample throughput capable of screening 96 samples per run, making the technique extremely time efficient. The RT-PCR-ELISA is a safe, quick, reliable technique, which has the potential for use as a standard virus detection method. (c) 2005 Elsevier B.V. All rights reserved.

AB - A new method, termed RT-PCR-ELISA, was evaluated for ease of use, reliability and sensitivity when detecting infectious pancreatic necrosis virus (IPNV) present in trout kidney tissue. The method had comparable sensitivity to existing PCR assays and could successfully detect 1.5 x 10(4) pfu IPNV in artificially contaminated trout kidney samples. The technique was easily established in a new laboratory and required no specialised equipment. The method had a high sample throughput capable of screening 96 samples per run, making the technique extremely time efficient. The RT-PCR-ELISA is a safe, quick, reliable technique, which has the potential for use as a standard virus detection method. (c) 2005 Elsevier B.V. All rights reserved.

KW - IPNV

KW - detection

KW - RT-PCR

KW - ELISA

KW - reverse-transcriptase PCR

KW - hepatitis-A virus

KW - ennteric viruses

KW - assay

U2 - 10.1016/j.jviromet.2005.09.003

DO - 10.1016/j.jviromet.2005.09.003

M3 - Article

VL - 132

SP - 92

EP - 96

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout

Abstract

Keywords

UN SDGs

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