A sensitive microsphere coagulation ELISA for Escherichia coli O157 : H7 using Russell's viper venom

N J C  Strachan; I D  Ogden

doi:10.1016/S0378-1097(00)00120-8

A sensitive microsphere coagulation ELISA for Escherichia coli O157 : H7 using Russell's viper venom

N J C Strachan, I D Ogden

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Abstract

A microsphere coagulation enzyme-linked immunosorbent assay (MC-ELISA) using Russell's viper venom factor X activator (RVV-XA) is described for the detection of Escherichia coli O157:H7. This microtitre plate assay comprises a standard sandwich immunoassay incorporating RVV-XA as the enzyme label. Coagulation substrate together with polystyrene microspheres are added to the wells of the microtitre plate. RVV-XA initiates the coagulation cascade causing formation of an artificial clot of polystyrene microspheres bound together with fibrin. As few as 10(2) E. coli O157 in a well (10(3) per ml) can be detected within 3 h. The assay is two orders of magnitude more sensitive than a standard ELISA and is a generic technique with the potential for widespread use in sandwich immunoassays. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	79-84
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	186
DOIs	https://doi.org/10.1016/S0378-1097(00)00120-8
Publication status	Published - 2000

Keywords

enzyme-linked immunosorbent assay
Russell's viper venom
Escherichia coli O157 : H7
HEMOLYTIC-UREMIC SYNDROME
ASSAY
OUTBREAK
SAMPLES
FOODS

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10.1016/S0378-1097(00)00120-8

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title = "A sensitive microsphere coagulation ELISA for Escherichia coli O157 : H7 using Russell's viper venom",

abstract = "A microsphere coagulation enzyme-linked immunosorbent assay (MC-ELISA) using Russell's viper venom factor X activator (RVV-XA) is described for the detection of Escherichia coli O157:H7. This microtitre plate assay comprises a standard sandwich immunoassay incorporating RVV-XA as the enzyme label. Coagulation substrate together with polystyrene microspheres are added to the wells of the microtitre plate. RVV-XA initiates the coagulation cascade causing formation of an artificial clot of polystyrene microspheres bound together with fibrin. As few as 10(2) E. coli O157 in a well (10(3) per ml) can be detected within 3 h. The assay is two orders of magnitude more sensitive than a standard ELISA and is a generic technique with the potential for widespread use in sandwich immunoassays. (C) 2000 Published by Elsevier Science B.V. All rights reserved.",

keywords = "enzyme-linked immunosorbent assay, Russell's viper venom, Escherichia coli O157 : H7, HEMOLYTIC-UREMIC SYNDROME, ASSAY, OUTBREAK, SAMPLES, FOODS",

author = "Strachan, {N J C} and Ogden, {I D}",

year = "2000",

doi = "10.1016/S0378-1097(00)00120-8",

language = "English",

volume = "186",

pages = "79--84",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

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TY - JOUR

T1 - A sensitive microsphere coagulation ELISA for Escherichia coli O157 : H7 using Russell's viper venom

AU - Strachan, N J C

AU - Ogden, I D

PY - 2000

Y1 - 2000

N2 - A microsphere coagulation enzyme-linked immunosorbent assay (MC-ELISA) using Russell's viper venom factor X activator (RVV-XA) is described for the detection of Escherichia coli O157:H7. This microtitre plate assay comprises a standard sandwich immunoassay incorporating RVV-XA as the enzyme label. Coagulation substrate together with polystyrene microspheres are added to the wells of the microtitre plate. RVV-XA initiates the coagulation cascade causing formation of an artificial clot of polystyrene microspheres bound together with fibrin. As few as 10(2) E. coli O157 in a well (10(3) per ml) can be detected within 3 h. The assay is two orders of magnitude more sensitive than a standard ELISA and is a generic technique with the potential for widespread use in sandwich immunoassays. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

AB - A microsphere coagulation enzyme-linked immunosorbent assay (MC-ELISA) using Russell's viper venom factor X activator (RVV-XA) is described for the detection of Escherichia coli O157:H7. This microtitre plate assay comprises a standard sandwich immunoassay incorporating RVV-XA as the enzyme label. Coagulation substrate together with polystyrene microspheres are added to the wells of the microtitre plate. RVV-XA initiates the coagulation cascade causing formation of an artificial clot of polystyrene microspheres bound together with fibrin. As few as 10(2) E. coli O157 in a well (10(3) per ml) can be detected within 3 h. The assay is two orders of magnitude more sensitive than a standard ELISA and is a generic technique with the potential for widespread use in sandwich immunoassays. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

KW - enzyme-linked immunosorbent assay

KW - Russell's viper venom

KW - Escherichia coli O157 : H7

KW - HEMOLYTIC-UREMIC SYNDROME

KW - ASSAY

KW - OUTBREAK

KW - SAMPLES

KW - FOODS

UR - http://www.scopus.com/inward/record.url?scp=0034194617&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(00)00120-8

DO - 10.1016/S0378-1097(00)00120-8

M3 - Article

C2 - 10779716

SN - 0378-1097

VL - 186

SP - 79

EP - 84

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

ER -

A sensitive microsphere coagulation ELISA for Escherichia coli O157 : H7 using Russell's viper venom

Abstract

Keywords

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