Abstract
A time-resolved immunofluorometric assay (TR-IFMA) for C-reactive protein (CRP) determination in whole blood of pigs was developed and validated. CRP was isolated from porcine acute-phase serum by affinity chromatography on agarose, coupled with phosphorylethanolamine and polyclonal antibodies to porcine CRP were purified from antiserum raised in sheep immunized with porcine CRP. Intra- and inter-assay coefficients of variation (CVs) were in the range 3.13-7.19% and 7.06-15.66%, respectively, showing good precision. The assay measured the CRP values in a proportional and linear manner (r=0.99); additionally, CRP concentrations measured in whole blood by the present TR-IFMA and in serum by an established immunoturbidimetric assay were highly correlated (R(2)=0.97). The limit of detection of the method was 0.0028 mg/L. Significantly lower CRP concentrations were observed after 7 days of sample storage at 4 degrees C. The injection of turpentine oil caused a significant increase in CRP concentrations and significantly higher CRP concentrations were observed in pigs with pathological processes compared to healthy animals.
Original language | English |
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Pages (from-to) | 171-176 |
Number of pages | 6 |
Journal | Luminescence |
Volume | 22 |
Issue number | 3 |
Early online date | 29 Jan 2007 |
DOIs | |
Publication status | Published - May 2007 |
Keywords
- animals
- blood
- C-reactive protein
- electrophoresis, polyacrylamide gel
- fluorescent antibody technique
- reproducibility of results
- sensitivity and specificity
- swine
- TR-IFMA
- whole blood
- pigs