An Antarctic molluscan biomineralisation tool-kit

Victoria A. Sleight; Benjamin Marie; Daniel J. Jackson; Elisabeth A. Dyrynda; Arul Marie; Melody S. Clark

doi:10.1038/srep36978

An Antarctic molluscan biomineralisation tool-kit

Victoria A. Sleight^* (Corresponding Author), Benjamin Marie, Daniel J. Jackson, Elisabeth A. Dyrynda, Arul Marie, Melody S. Clark

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

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Abstract

The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, proteomics and gene expression to investigate this process. Thirty seven proteins were identified via proteomic extraction of the nacreous shell layer, including two not previously found in nacre; a novel T-rich Mucin-like protein and a Zinc-dependent metalloprotease. In situ hybridisation of seven candidate biomineralisation genes revealed discrete spatial expression patterns within the mantle tissue, hinting at modular organisation, which is also observed in the mantle tissues of other molluscs. All seven of these biomineralisation candidates displayed evidence of multifunctionality and strong association with vesicles, which are potentially involved in shell secretion in this species.

Original language	English
Article number	36978
Number of pages	13
Journal	Scientific Reports
Volume	6
Early online date	11 Nov 2016
DOIs	https://doi.org/10.1038/srep36978
Publication status	Published - 11 Nov 2016

Bibliographical note

Acknowledgements
VAS was funded by a NERC DTG studentship (Project Reference: NE/J500173/1) to the British Antarctic Survey. MSC was financed by NERC core funding to the British Antarctic Survey. We would like to thank Jeremy Skepper for advising us on TEM procedures and processing the mantle tissue samples prior to TEM, Elizabeth M. Harper for advice on Laternula elliptica mantle anatomy for the schematic, Jamie Oliver for technical help with digitising the schematic, Deborah M. Power for in situ hybridisation advice, Valerie J. Smith for advice on immunology and histology, Lloyd S. Peck for vesicle discussions and critical reading of the manuscript and the dive team at Rothera research station, Antarctica for support in animal collection. Diving oversight was provided by the NERC National Facility for Scientific Diving, Oban.

Keywords

environmental sciences
gene expression
proteomics
zoology

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Cite this

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title = "An Antarctic molluscan biomineralisation tool-kit",

abstract = "The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, proteomics and gene expression to investigate this process. Thirty seven proteins were identified via proteomic extraction of the nacreous shell layer, including two not previously found in nacre; a novel T-rich Mucin-like protein and a Zinc-dependent metalloprotease. In situ hybridisation of seven candidate biomineralisation genes revealed discrete spatial expression patterns within the mantle tissue, hinting at modular organisation, which is also observed in the mantle tissues of other molluscs. All seven of these biomineralisation candidates displayed evidence of multifunctionality and strong association with vesicles, which are potentially involved in shell secretion in this species.",

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An Antarctic molluscan biomineralisation tool-kit

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Victoria Sleight

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An Antarctic molluscan biomineralisation tool-kit

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Bibliographical note

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Victoria Sleight

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