Aorta protein networks in marginal and acute zinc deficiency

John Hamilton Beattie, Margaret Jane Gordon, Garry Jonathan Rucklidge, Martin David Reid, Gary James Duncan, Graham W Horgan, Young-Eun Cho, In-Sook Kwun

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Human zinc deficiency is a global problem and may influence the development of cardiovascular disease. Our objective was to determine Zn deficiency affected pathways and protein interactions in rat aorta and their likely influence on stress-induced atherogenesis. In two separate studies, rats were given diets acutely (< 1 mg Zn/kg) or marginally (6 mg Zn/kg) deficient in Zn. Both studies included Zn adequate controls (35 mg Zn/kg) and the acute deficiency study included a pair-fed group. After 6 wk, proteins from thoracic aorta were separated by 2-DE. Proteins affected by zinc deficiency were identified by principal component analysis. Multiple correlations of identified proteins indicated protein networks of related function. Proteins clusters decreased in zinc deficiency were related to fatty acid and carbohydrate metabolism. Structurally related proteins, including zyxin and over nine transgelin 1 proteins, were either increased or decreased by acute and marginal deficiencies. PKC alpha was significantly decreased in Zn deficiency suggesting that Zn may regulate the phosphorylation of target proteins. Zn deficiency-related changes in structural, carbohydrate and fatty acid-related proteins may be disadvantageous for maintaining vascular health and are consistent with a protective role for zinc in the development of atherosclerosis.

Original languageEnglish
Pages (from-to)2126-2135
Number of pages10
Issue number10
Publication statusPublished - May 2008


  • atherosclerosis
  • transgelin
  • vascular health
  • zinc deficiency
  • vascular smooth-muscle
  • glycolytic-enzymes
  • endothelial-cells
  • single nutrient
  • blood-pressure
  • target genes
  • coconut oil
  • lim domain
  • kinase-C
  • rats
  • Atherosclerosis
  • Transgelin
  • Vascular health
  • Zinc deficiency


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