Abstract
Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.
Original language | English |
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Pages (from-to) | 432-437 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 299 |
Issue number | 3 |
DOIs | |
Publication status | Published - 6 Dec 2002 |
Keywords
- RECEPTOR MESSENGER-RNA
- FATTY-ACIDS
- OBESE GENE
- BINDING PROTEIN
- DIABETIC MICE
- OB-R
- METABOLISM
- EXPRESSION
- LOCALIZATION
- INTERNALIZATION
- receptor messenger-RNA
- fatty-acids
- obese gene
- bindingf protein
- diabetic mice
- metabolism
- expression
- localization
- internalization