Assessment of acute PAH toxicity using prokaryote biosensors.

B J  Reid; C J A  MacLeod; K T  Semple; H J  Weitz; G I  Paton

Assessment of acute PAH toxicity using prokaryote biosensors.

B J Reid, C J A MacLeod, K T Semple, H J Weitz, G I Paton

Research output: Chapter in Book/Report/Conference proceeding › Published conference contribution

Abstract

The aim of this study was to assess the acute toxicity of phenanthrene, pyrene and benzo[a]pyrene using fur-marked bacterial biosensors. Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl cyclodextrin (HPCD) solution. Four microorganisms containing the lux cassette were used as the test biosensors and over the incubation time period (280 min), there was no significant decrease in bioluminescence in any of the biosensors. This study has shown that the three PAHs tested are not acutely toxic to the prokaryotic biosensors, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic compounds, such as PAHs, from more complex environmental matrices.

Original language	English
Title of host publication	Bioremediation Technologies for Polycyclic Aromatic Hydrocarbon Compounds
Editors	A Leeson, BC Alleman
Place of Publication	Columbus
Publisher	Battelle Press
Pages	217-222
Number of pages	6
ISBN (Print)	1-57477-081-0
Publication status	Published - 1999
Event	5th International In Situ and On-Site Bioremediation Symposium - SAN DIEGO, Canada Duration: 19 Apr 1999 → 22 Apr 1999

Conference

Conference	5th International In Situ and On-Site Bioremediation Symposium
Country/Territory	Canada
City	SAN DIEGO
Period	19/04/99 → 22/04/99

Keywords

BIOAVAILABILITY
SOIL

Cite this

@inproceedings{72b3f6b2d36a472280c3ea7fedc40963,

title = "Assessment of acute PAH toxicity using prokaryote biosensors.",

abstract = "The aim of this study was to assess the acute toxicity of phenanthrene, pyrene and benzo[a]pyrene using fur-marked bacterial biosensors. Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl cyclodextrin (HPCD) solution. Four microorganisms containing the lux cassette were used as the test biosensors and over the incubation time period (280 min), there was no significant decrease in bioluminescence in any of the biosensors. This study has shown that the three PAHs tested are not acutely toxic to the prokaryotic biosensors, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic compounds, such as PAHs, from more complex environmental matrices.",

keywords = "BIOAVAILABILITY, SOIL",

author = "Reid, {B J} and MacLeod, {C J A} and Semple, {K T} and Weitz, {H J} and Paton, {G I}",

year = "1999",

language = "English",

isbn = "1-57477-081-0",

pages = "217--222",

editor = "A Leeson and BC Alleman",

booktitle = "Bioremediation Technologies for Polycyclic Aromatic Hydrocarbon Compounds",

publisher = "Battelle Press",

note = "5th International In Situ and On-Site Bioremediation Symposium ; Conference date: 19-04-1999 Through 22-04-1999",

}

TY - GEN

T1 - Assessment of acute PAH toxicity using prokaryote biosensors.

AU - Reid, B J

AU - MacLeod, C J A

AU - Semple, K T

AU - Weitz, H J

AU - Paton, G I

PY - 1999

Y1 - 1999

N2 - The aim of this study was to assess the acute toxicity of phenanthrene, pyrene and benzo[a]pyrene using fur-marked bacterial biosensors. Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl cyclodextrin (HPCD) solution. Four microorganisms containing the lux cassette were used as the test biosensors and over the incubation time period (280 min), there was no significant decrease in bioluminescence in any of the biosensors. This study has shown that the three PAHs tested are not acutely toxic to the prokaryotic biosensors, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic compounds, such as PAHs, from more complex environmental matrices.

AB - The aim of this study was to assess the acute toxicity of phenanthrene, pyrene and benzo[a]pyrene using fur-marked bacterial biosensors. Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl cyclodextrin (HPCD) solution. Four microorganisms containing the lux cassette were used as the test biosensors and over the incubation time period (280 min), there was no significant decrease in bioluminescence in any of the biosensors. This study has shown that the three PAHs tested are not acutely toxic to the prokaryotic biosensors, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic compounds, such as PAHs, from more complex environmental matrices.

KW - BIOAVAILABILITY

KW - SOIL

M3 - Published conference contribution

SN - 1-57477-081-0

SP - 217

EP - 222

BT - Bioremediation Technologies for Polycyclic Aromatic Hydrocarbon Compounds

A2 - Leeson, A

A2 - Alleman, BC

PB - Battelle Press

CY - Columbus

T2 - 5th International In Situ and On-Site Bioremediation Symposium

Y2 - 19 April 1999 through 22 April 1999

ER -

Assessment of acute PAH toxicity using prokaryote biosensors.

Abstract

Conference

Keywords

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