Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

Sara Saffar Soflaei; Mojtaba Baktashian; Kiana Hosseinpour Moghaddam; Maryam Saberi-Karimian; Negin Kosari; Seyed Mohammad Hashemi; Mohsen Mouhebati; Mahsa Amini; Mashallah Dehghani; Habibollah Esmaily; Mahmoud Ebrahimi; Homa Falsoleiman; Abolfazl Nosrati-Tirkani; Fatemeh Sadabadi; Gordon A. Ferns; Mansoor Salehi; Alireza Pasdar; Majid Ghayour-Mobarhan

doi:10.36660/abc.20210422

Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

Translated title of the contribution: Association of Paraoxonase-1 Genotype and Phenotype with Angiogram Positive Coronary Artery Disease

Sara Saffar Soflaei, Mojtaba Baktashian, Kiana Hosseinpour Moghaddam, Maryam Saberi-Karimian, Negin Kosari, Seyed Mohammad Hashemi, Mohsen Mouhebati, Mahsa Amini, Mashallah Dehghani, Habibollah Esmaily, Mahmoud Ebrahimi, Homa Falsoleiman, Abolfazl Nosrati-Tirkani, Fatemeh Sadabadi, Gordon A. Ferns, Mansoor Salehi, Alireza Pasdar, Majid Ghayour-Mobarhan^*

^*Corresponding author for this work

Applied Medicine

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

BACKGROUND: It has been shown that increased serum PON1 levels are protective against several disorders. Several single nucleotide polymorphisms (SNPs) of the PON1 gene have been reported to be associated with serum enzyme protein levels and activity.

OBJECTIVE: To investigate the association of SNPs of PON1 and serum paraoxonase activity with coronary artery disease (CAD).

METHODS: A total of 601 unrelated patients who underwent coronary angiography including those who had >50% stenosis (N=266) and those with <30% stenosis (N=335) were studied. The Paraoxonase gene rs662 and rs840560 SNPs were determined using the ARMS-PCR method and the rs705379 SNP was genotyped using PCR-RFLP analysis. Serum paraoxonase activity was measured using paraoxon as a substrate. A p value of p<0.05 was considered as significant.

RESULTS: Serum paraoxonase activity was not significantly different between the study groups. After adjustment for age, sex, hypertension, diabetes mellitus and dyslipidemia, the GG genotype and co-dominant model of rs662 was positively associated with a positive angiogram (respectively, OR=2.424, 95%CI [1.123-5.233], p<0.05, OR=1.663, 95%CI [1.086-2.547]). Serum paraoxonase activity was significantly higher in the G allele and GG variant of rs662, A allele and AA variant of rs854560 and C allele and CC variant of rs705379. The haplotype analysis has shown that the ATC haplotype was significantly more prevalent among the angiogram negative group. The analysis between groups indicated that the A allele of rs662 was significantly associated with lower paraoxonase activity in the positive angiogram group (p=0.019).

CONCLUSIONS: The presence of the G allele of the rs662 single nucleotide polymorphism is independently associated to increased risk of CAD.

Translated title of the contribution	Association of Paraoxonase-1 Genotype and Phenotype with Angiogram Positive Coronary Artery Disease
Original language	Portuguese
Pages (from-to)	593-601
Number of pages	9
Journal	Arquivos Brasileiros de Cardiologia
Volume	119
Issue number	4
DOIs	https://doi.org/10.36660/abc.20210422
Publication status	Published - 29 Aug 2022

Bibliographical note

Funding Information:
This study was supported by Mashhad and Isfahan University of Medical Sciences. The authors would like to thank technicians of Sina, Sadi, Ghaem catheterization laboratory and technicians of Isfahan Alzahra genetics laboratory.

Keywords

Aryldialkylphosphatase/genetics
Angiografia
doenca da arteria coronariana

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Soflaei, S. S., Baktashian, M., Moghaddam, K. H., Saberi-Karimian, M., Kosari, N., Hashemi, S. M., Mouhebati, M., Amini, M., Dehghani, M., Esmaily, H., Ebrahimi, M., Falsoleiman, H., Nosrati-Tirkani, A., Sadabadi, F., Ferns, G. A., Salehi, M., Pasdar, A., & Ghayour-Mobarhan, M. (2022). Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana. Arquivos Brasileiros de Cardiologia, 119(4), 593-601. https://doi.org/10.36660/abc.20210422

Soflaei, SS, Baktashian, M, Moghaddam, KH, Saberi-Karimian, M, Kosari, N, Hashemi, SM, Mouhebati, M, Amini, M, Dehghani, M, Esmaily, H, Ebrahimi, M, Falsoleiman, H, Nosrati-Tirkani, A, Sadabadi, F, Ferns, GA, Salehi, M, Pasdar, A & Ghayour-Mobarhan, M 2022, 'Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana', Arquivos Brasileiros de Cardiologia, vol. 119, no. 4, pp. 593-601. https://doi.org/10.36660/abc.20210422

@article{4e396819cb7145c28da347111f36f26c,

title = "Associa{\c c}{\~a}o do Gen{\'o}tipo e Fen{\'o}tipo da Paraoxonase-1 com Angiografia Positiva para Doen{\c c}a Arterial Coronariana",

abstract = "BACKGROUND: It has been shown that increased serum PON1 levels are protective against several disorders. Several single nucleotide polymorphisms (SNPs) of the PON1 gene have been reported to be associated with serum enzyme protein levels and activity.OBJECTIVE: To investigate the association of SNPs of PON1 and serum paraoxonase activity with coronary artery disease (CAD).METHODS: A total of 601 unrelated patients who underwent coronary angiography including those who had >50% stenosis (N=266) and those with <30% stenosis (N=335) were studied. The Paraoxonase gene rs662 and rs840560 SNPs were determined using the ARMS-PCR method and the rs705379 SNP was genotyped using PCR-RFLP analysis. Serum paraoxonase activity was measured using paraoxon as a substrate. A p value of p<0.05 was considered as significant.RESULTS: Serum paraoxonase activity was not significantly different between the study groups. After adjustment for age, sex, hypertension, diabetes mellitus and dyslipidemia, the GG genotype and co-dominant model of rs662 was positively associated with a positive angiogram (respectively, OR=2.424, 95%CI [1.123-5.233], p<0.05, OR=1.663, 95%CI [1.086-2.547]). Serum paraoxonase activity was significantly higher in the G allele and GG variant of rs662, A allele and AA variant of rs854560 and C allele and CC variant of rs705379. The haplotype analysis has shown that the ATC haplotype was significantly more prevalent among the angiogram negative group. The analysis between groups indicated that the A allele of rs662 was significantly associated with lower paraoxonase activity in the positive angiogram group (p=0.019).CONCLUSIONS: The presence of the G allele of the rs662 single nucleotide polymorphism is independently associated to increased risk of CAD.",

keywords = "Aryldialkylphosphatase/genetics, Angiografia, doenca da arteria coronariana",

author = "Soflaei, {Sara Saffar} and Mojtaba Baktashian and Moghaddam, {Kiana Hosseinpour} and Maryam Saberi-Karimian and Negin Kosari and Hashemi, {Seyed Mohammad} and Mohsen Mouhebati and Mahsa Amini and Mashallah Dehghani and Habibollah Esmaily and Mahmoud Ebrahimi and Homa Falsoleiman and Abolfazl Nosrati-Tirkani and Fatemeh Sadabadi and Ferns, {Gordon A.} and Mansoor Salehi and Alireza Pasdar and Majid Ghayour-Mobarhan",

note = "Funding Information: This study was supported by Mashhad and Isfahan University of Medical Sciences. The authors would like to thank technicians of Sina, Sadi, Ghaem catheterization laboratory and technicians of Isfahan Alzahra genetics laboratory. ",

year = "2022",

month = aug,

day = "29",

doi = "10.36660/abc.20210422",

language = "Portuguese",

volume = "119",

pages = "593--601",

journal = "Arquivos Brasileiros de Cardiologia",

issn = "0066-782X",

publisher = "Arquivos Brasileiros de Cardiologia",

number = "4",

}

TY - JOUR

T1 - Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

AU - Soflaei, Sara Saffar

AU - Baktashian, Mojtaba

AU - Moghaddam, Kiana Hosseinpour

AU - Saberi-Karimian, Maryam

AU - Kosari, Negin

AU - Hashemi, Seyed Mohammad

AU - Mouhebati, Mohsen

AU - Amini, Mahsa

AU - Dehghani, Mashallah

AU - Esmaily, Habibollah

AU - Ebrahimi, Mahmoud

AU - Falsoleiman, Homa

AU - Nosrati-Tirkani, Abolfazl

AU - Sadabadi, Fatemeh

AU - Ferns, Gordon A.

AU - Salehi, Mansoor

AU - Pasdar, Alireza

AU - Ghayour-Mobarhan, Majid

N1 - Funding Information: This study was supported by Mashhad and Isfahan University of Medical Sciences. The authors would like to thank technicians of Sina, Sadi, Ghaem catheterization laboratory and technicians of Isfahan Alzahra genetics laboratory.

PY - 2022/8/29

Y1 - 2022/8/29

N2 - BACKGROUND: It has been shown that increased serum PON1 levels are protective against several disorders. Several single nucleotide polymorphisms (SNPs) of the PON1 gene have been reported to be associated with serum enzyme protein levels and activity.OBJECTIVE: To investigate the association of SNPs of PON1 and serum paraoxonase activity with coronary artery disease (CAD).METHODS: A total of 601 unrelated patients who underwent coronary angiography including those who had >50% stenosis (N=266) and those with <30% stenosis (N=335) were studied. The Paraoxonase gene rs662 and rs840560 SNPs were determined using the ARMS-PCR method and the rs705379 SNP was genotyped using PCR-RFLP analysis. Serum paraoxonase activity was measured using paraoxon as a substrate. A p value of p<0.05 was considered as significant.RESULTS: Serum paraoxonase activity was not significantly different between the study groups. After adjustment for age, sex, hypertension, diabetes mellitus and dyslipidemia, the GG genotype and co-dominant model of rs662 was positively associated with a positive angiogram (respectively, OR=2.424, 95%CI [1.123-5.233], p<0.05, OR=1.663, 95%CI [1.086-2.547]). Serum paraoxonase activity was significantly higher in the G allele and GG variant of rs662, A allele and AA variant of rs854560 and C allele and CC variant of rs705379. The haplotype analysis has shown that the ATC haplotype was significantly more prevalent among the angiogram negative group. The analysis between groups indicated that the A allele of rs662 was significantly associated with lower paraoxonase activity in the positive angiogram group (p=0.019).CONCLUSIONS: The presence of the G allele of the rs662 single nucleotide polymorphism is independently associated to increased risk of CAD.

AB - BACKGROUND: It has been shown that increased serum PON1 levels are protective against several disorders. Several single nucleotide polymorphisms (SNPs) of the PON1 gene have been reported to be associated with serum enzyme protein levels and activity.OBJECTIVE: To investigate the association of SNPs of PON1 and serum paraoxonase activity with coronary artery disease (CAD).METHODS: A total of 601 unrelated patients who underwent coronary angiography including those who had >50% stenosis (N=266) and those with <30% stenosis (N=335) were studied. The Paraoxonase gene rs662 and rs840560 SNPs were determined using the ARMS-PCR method and the rs705379 SNP was genotyped using PCR-RFLP analysis. Serum paraoxonase activity was measured using paraoxon as a substrate. A p value of p<0.05 was considered as significant.RESULTS: Serum paraoxonase activity was not significantly different between the study groups. After adjustment for age, sex, hypertension, diabetes mellitus and dyslipidemia, the GG genotype and co-dominant model of rs662 was positively associated with a positive angiogram (respectively, OR=2.424, 95%CI [1.123-5.233], p<0.05, OR=1.663, 95%CI [1.086-2.547]). Serum paraoxonase activity was significantly higher in the G allele and GG variant of rs662, A allele and AA variant of rs854560 and C allele and CC variant of rs705379. The haplotype analysis has shown that the ATC haplotype was significantly more prevalent among the angiogram negative group. The analysis between groups indicated that the A allele of rs662 was significantly associated with lower paraoxonase activity in the positive angiogram group (p=0.019).CONCLUSIONS: The presence of the G allele of the rs662 single nucleotide polymorphism is independently associated to increased risk of CAD.

KW - Aryldialkylphosphatase/genetics

KW - Angiografia

KW - doenca da arteria coronariana

UR - http://www.scopus.com/inward/record.url?scp=85140040722&partnerID=8YFLogxK

U2 - 10.36660/abc.20210422

DO - 10.36660/abc.20210422

M3 - Article

C2 - 36074479

AN - SCOPUS:85140040722

SN - 0066-782X

VL - 119

SP - 593

EP - 601

JO - Arquivos Brasileiros de Cardiologia

JF - Arquivos Brasileiros de Cardiologia

IS - 4

ER -

Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

Abstract

Bibliographical note

Keywords

UN SDGs

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