TY - JOUR
T1 - Association of Interleukin-10 –592 C > A gene polymorphism with coronary artery disease
T2 - A case-control study and meta-analysis
AU - Ghalandari, Marzieh
AU - Jamialahmadi, Khadijeh
AU - Nik, Maryam Mardan
AU - Pirhoushiaran, Maryam
AU - Mirhafez, Seyed Reza
AU - Rooki, Hassan
AU - Avan, Amir
AU - Ghazizadeh, Hamideh
AU - Moohebati, Mohsen
AU - Nohtani, Mahdi
AU - Zaimkohan, Hooshang
AU - Ferns, Gordon A.
AU - Pasdar, Alireza
AU - Ghayour-Mobarhan, Majid
N1 - Funding Information:
We would like to thank Mashhad University of Medical Sciences Research council for their financial support.
PY - 2021/3/1
Y1 - 2021/3/1
N2 - Background: Coronary-artery-disease (CAD) is the leading cause of death worldwide, and hence there is a need to identify reliable markers for identifying individuals at high risk of developing CAD. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is associated with an increased risk of developing both atherosclerosis and acute coronary events. The study aimed to explore the association of a genetic variant in IL-10 with the risk of developing CAD and the severity of the disease. To further explore, a systematic review and meta-analysis was performed. The cumulative results of the relationship between IL and 10 –592 C > A polymorphism and CAD in Iranian population have also been presented. Methods: In this cross sectional study, a total of 948 individuals including 307 healthy controls and 641 patients that among cases, four hundred and fifty-five of the patients had > 50% stenosis (angiogram positive group) and 186 patients had < 50% stenosis (angiogram negative group) were recruited from the Mashhad-Stroke and Heart-Atherosclerotic-Disorders cohort. Genotyping for the IL-10 –592 C > A polymorphism was performed using a PCR-RFLP technique, and statistical analysis undertaken by univariate and multivariate analyses. PubMed, Google Scholar and Scopus were searched for papers related to this polymorphism up to October 2019. The Meta-analysis was done based on the random effect model using a Meta-analysis. Results: In our study, the frequency of the variant A allele of the IL-10 –592 C > A was significantly higher in CAD patients than the control group (P value = 0.043). Moreover, subjects carrying AA genotype had a significantly higher risk of CAD (OR: 1.8, 95%CI: 1.04–3.16), p = 0.03), compared to those with the wild type genotype. The results of meta-analysis of 9336 cases and 8461 controls did not also show any significant association between IL and 10 –592 C > A and CAD in dominant and recessive genetic models but only in co-dominant model when fix effect was applied. Conclusion: Although our research findings support a significant association of genetic polymorphism in the IL10 gene with cardiovascular diseases, this finding cannot be confirmed in meta-analysis. Further functional analysis and evaluation of this marker in a multicenter setting are needed to establish its value as a risk stratification marker.
AB - Background: Coronary-artery-disease (CAD) is the leading cause of death worldwide, and hence there is a need to identify reliable markers for identifying individuals at high risk of developing CAD. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is associated with an increased risk of developing both atherosclerosis and acute coronary events. The study aimed to explore the association of a genetic variant in IL-10 with the risk of developing CAD and the severity of the disease. To further explore, a systematic review and meta-analysis was performed. The cumulative results of the relationship between IL and 10 –592 C > A polymorphism and CAD in Iranian population have also been presented. Methods: In this cross sectional study, a total of 948 individuals including 307 healthy controls and 641 patients that among cases, four hundred and fifty-five of the patients had > 50% stenosis (angiogram positive group) and 186 patients had < 50% stenosis (angiogram negative group) were recruited from the Mashhad-Stroke and Heart-Atherosclerotic-Disorders cohort. Genotyping for the IL-10 –592 C > A polymorphism was performed using a PCR-RFLP technique, and statistical analysis undertaken by univariate and multivariate analyses. PubMed, Google Scholar and Scopus were searched for papers related to this polymorphism up to October 2019. The Meta-analysis was done based on the random effect model using a Meta-analysis. Results: In our study, the frequency of the variant A allele of the IL-10 –592 C > A was significantly higher in CAD patients than the control group (P value = 0.043). Moreover, subjects carrying AA genotype had a significantly higher risk of CAD (OR: 1.8, 95%CI: 1.04–3.16), p = 0.03), compared to those with the wild type genotype. The results of meta-analysis of 9336 cases and 8461 controls did not also show any significant association between IL and 10 –592 C > A and CAD in dominant and recessive genetic models but only in co-dominant model when fix effect was applied. Conclusion: Although our research findings support a significant association of genetic polymorphism in the IL10 gene with cardiovascular diseases, this finding cannot be confirmed in meta-analysis. Further functional analysis and evaluation of this marker in a multicenter setting are needed to establish its value as a risk stratification marker.
KW - Coronary artery disease
KW - Inflammation, Meta-analysis
KW - Interleukin-10 –592 C > A polymorphism
UR - http://www.scopus.com/inward/record.url?scp=85100037650&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2020.155403
DO - 10.1016/j.cyto.2020.155403
M3 - Article
C2 - 33472122
AN - SCOPUS:85100037650
SN - 1043-4666
VL - 139
JO - Cytokine
JF - Cytokine
M1 - 155403
ER -
