Blood pressure and heart rate QTL in mice of the B6/D2 lineage: sex differences and environmental influences

David A Blizard; Arimantas Lionikas; David J Vandenbergh; Terrie Vasilopoulos; Glenn S Gerhard; James W Griffith; Laura C Klein; Joseph T Stout; Holly A Mack; Joan M Lakoski; Lars Larsson; Jeanne M Spicer; George P Vogler; Gerald E McClearn

doi:10.1152/physiolgenomics.00035.2008

Blood pressure and heart rate QTL in mice of the B6/D2 lineage: sex differences and environmental influences

David A Blizard, Arimantas Lionikas, David J Vandenbergh, Terrie Vasilopoulos, Glenn S Gerhard, James W Griffith, Laura C Klein, Joseph T Stout, Holly A Mack, Joan M Lakoski, Lars Larsson, Jeanne M Spicer, George P Vogler, Gerald E McClearn

Medical Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A quantitative trait locus (QTL) approach was used to define the genetic architecture underlying variation in systolic blood pressure (SBP) and heart rate (HR), measured indirectly on seven occasions by the tail cuff procedure. The tests were conducted in 395 F(2) adult mice (197 males, 198 females) derived from a cross of the C57BL/6J (B6) and DBA/2J (D2) strains and in 22 BXD recombinant-inbred (RI) strains. Interval mapping of F(2) data for the first 5 days of measurement nominated one statistically significant and one suggestive QTL for SBP on chromosomes (Chr) 4 and 14, respectively, and two statistically significant QTL for HR on Chr 1 (which was specific to female mice) and Chr 5. New suggestive QTL emerged for SBP on Chr 3 (female-specific) and 8 and for HR on Chr 11 for measurements recorded several weeks after mice had undergone stressful blood sampling procedures. The two statistically significant HR QTL were confirmed by analyses of BXD RI strain means. Male and female F(2) mice did not differ in SBP or HR but RI strain analyses showed pronounced strain-by-sex interactions and a negative genetic correlation between the two measures in both sexes. Evidence for a role for mitochondrial DNA was found for both HR and SBP. QTL for HR and SBP may differ in males and females and may be sensitive to different environmental contexts.

Original language	English
Pages (from-to)	158-166
Number of pages	9
Journal	Physiological Genomics
Volume	36
Issue number	3
Early online date	9 Dec 2008
DOIs	https://doi.org/10.1152/physiolgenomics.00035.2008
Publication status	Published - Feb 2009

Keywords

animals
blood pressure
chromosome mapping
chromosomes, mammalian
epistasis, genetic
female
genotype
heart rate
male
mice
mice, inbred C57BL
mice, inbred DBA
mice, inbred strains
polymorphism, single nucleotide
quantitative trait loci
sex factors
stress, physiological
quantitative trait locus

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10.1152/physiolgenomics.00035.2008

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Blizard, D. A., Lionikas, A., Vandenbergh, D. J., Vasilopoulos, T., Gerhard, G. S., Griffith, J. W., Klein, L. C., Stout, J. T., Mack, H. A., Lakoski, J. M., Larsson, L., Spicer, J. M., Vogler, G. P., & McClearn, G. E. (2009). Blood pressure and heart rate QTL in mice of the B6/D2 lineage: sex differences and environmental influences. Physiological Genomics, 36(3), 158-166. https://doi.org/10.1152/physiolgenomics.00035.2008

Blizard, DA, Lionikas, A, Vandenbergh, DJ, Vasilopoulos, T, Gerhard, GS, Griffith, JW, Klein, LC, Stout, JT, Mack, HA, Lakoski, JM, Larsson, L, Spicer, JM, Vogler, GP & McClearn, GE 2009, 'Blood pressure and heart rate QTL in mice of the B6/D2 lineage: sex differences and environmental influences', Physiological Genomics, vol. 36, no. 3, pp. 158-166. https://doi.org/10.1152/physiolgenomics.00035.2008

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abstract = "A quantitative trait locus (QTL) approach was used to define the genetic architecture underlying variation in systolic blood pressure (SBP) and heart rate (HR), measured indirectly on seven occasions by the tail cuff procedure. The tests were conducted in 395 F(2) adult mice (197 males, 198 females) derived from a cross of the C57BL/6J (B6) and DBA/2J (D2) strains and in 22 BXD recombinant-inbred (RI) strains. Interval mapping of F(2) data for the first 5 days of measurement nominated one statistically significant and one suggestive QTL for SBP on chromosomes (Chr) 4 and 14, respectively, and two statistically significant QTL for HR on Chr 1 (which was specific to female mice) and Chr 5. New suggestive QTL emerged for SBP on Chr 3 (female-specific) and 8 and for HR on Chr 11 for measurements recorded several weeks after mice had undergone stressful blood sampling procedures. The two statistically significant HR QTL were confirmed by analyses of BXD RI strain means. Male and female F(2) mice did not differ in SBP or HR but RI strain analyses showed pronounced strain-by-sex interactions and a negative genetic correlation between the two measures in both sexes. Evidence for a role for mitochondrial DNA was found for both HR and SBP. QTL for HR and SBP may differ in males and females and may be sensitive to different environmental contexts.",

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author = "Blizard, {David A} and Arimantas Lionikas and Vandenbergh, {David J} and Terrie Vasilopoulos and Gerhard, {Glenn S} and Griffith, {James W} and Klein, {Laura C} and Stout, {Joseph T} and Mack, {Holly A} and Lakoski, {Joan M} and Lars Larsson and Spicer, {Jeanne M} and Vogler, {George P} and McClearn, {Gerald E}",

year = "2009",

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doi = "10.1152/physiolgenomics.00035.2008",

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T1 - Blood pressure and heart rate QTL in mice of the B6/D2 lineage

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AU - Blizard, David A

AU - Lionikas, Arimantas

AU - Vandenbergh, David J

AU - Vasilopoulos, Terrie

AU - Gerhard, Glenn S

AU - Griffith, James W

AU - Klein, Laura C

AU - Stout, Joseph T

AU - Mack, Holly A

AU - Lakoski, Joan M

AU - Larsson, Lars

AU - Spicer, Jeanne M

AU - Vogler, George P

AU - McClearn, Gerald E

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N2 - A quantitative trait locus (QTL) approach was used to define the genetic architecture underlying variation in systolic blood pressure (SBP) and heart rate (HR), measured indirectly on seven occasions by the tail cuff procedure. The tests were conducted in 395 F(2) adult mice (197 males, 198 females) derived from a cross of the C57BL/6J (B6) and DBA/2J (D2) strains and in 22 BXD recombinant-inbred (RI) strains. Interval mapping of F(2) data for the first 5 days of measurement nominated one statistically significant and one suggestive QTL for SBP on chromosomes (Chr) 4 and 14, respectively, and two statistically significant QTL for HR on Chr 1 (which was specific to female mice) and Chr 5. New suggestive QTL emerged for SBP on Chr 3 (female-specific) and 8 and for HR on Chr 11 for measurements recorded several weeks after mice had undergone stressful blood sampling procedures. The two statistically significant HR QTL were confirmed by analyses of BXD RI strain means. Male and female F(2) mice did not differ in SBP or HR but RI strain analyses showed pronounced strain-by-sex interactions and a negative genetic correlation between the two measures in both sexes. Evidence for a role for mitochondrial DNA was found for both HR and SBP. QTL for HR and SBP may differ in males and females and may be sensitive to different environmental contexts.

AB - A quantitative trait locus (QTL) approach was used to define the genetic architecture underlying variation in systolic blood pressure (SBP) and heart rate (HR), measured indirectly on seven occasions by the tail cuff procedure. The tests were conducted in 395 F(2) adult mice (197 males, 198 females) derived from a cross of the C57BL/6J (B6) and DBA/2J (D2) strains and in 22 BXD recombinant-inbred (RI) strains. Interval mapping of F(2) data for the first 5 days of measurement nominated one statistically significant and one suggestive QTL for SBP on chromosomes (Chr) 4 and 14, respectively, and two statistically significant QTL for HR on Chr 1 (which was specific to female mice) and Chr 5. New suggestive QTL emerged for SBP on Chr 3 (female-specific) and 8 and for HR on Chr 11 for measurements recorded several weeks after mice had undergone stressful blood sampling procedures. The two statistically significant HR QTL were confirmed by analyses of BXD RI strain means. Male and female F(2) mice did not differ in SBP or HR but RI strain analyses showed pronounced strain-by-sex interactions and a negative genetic correlation between the two measures in both sexes. Evidence for a role for mitochondrial DNA was found for both HR and SBP. QTL for HR and SBP may differ in males and females and may be sensitive to different environmental contexts.

KW - animals

KW - blood pressure

KW - chromosome mapping

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KW - epistasis, genetic

KW - female

KW - genotype

KW - heart rate

KW - male

KW - mice

KW - mice, inbred C57BL

KW - mice, inbred DBA

KW - mice, inbred strains

KW - polymorphism, single nucleotide

KW - quantitative trait loci

KW - sex factors

KW - stress, physiological

KW - quantitative trait locus

U2 - 10.1152/physiolgenomics.00035.2008

DO - 10.1152/physiolgenomics.00035.2008

M3 - Article

C2 - 19066325

SN - 1094-8341

VL - 36

SP - 158

EP - 166

JO - Physiological Genomics

JF - Physiological Genomics

IS - 3

ER -

Blood pressure and heart rate QTL in mice of the B6/D2 lineage: sex differences and environmental influences

Abstract

Keywords

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