Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells

Adam T Lynch, Silvia Mazzotta, Stefan Hoppler

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)


In vitro generated mammalian cardiomyocytes provide experimental models for studying normal mammalian cardiomyocyte development, for disease modelling and for drug development. They also promise to inform future therapeutic strategies for repair of injured or diseased myocardium. Here we provide reliable protocols for differentiation of mouse embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimising such protocols for specific cell lines.
Original languageEnglish
Title of host publicationExperimental Models of Cardiovascular Disease
Subtitle of host publicationMethods and Protocols
EditorsK Ishikawa
Number of pages12
ISBN (Print)978-1-4939-8596-8
Publication statusE-pub ahead of print - 10 Jul 2018

Publication series

NameMethods in Molecular Biology Series
ISSN (Electronic)1064-3745

Bibliographical note

We thank the Bruneau lab (Gladstone Institute) and the Martinez-Arias lab (University of Cambridge) for helpful advice regarding mouse ES cell culture. We thank Kate Watt and Yvonne Turnbull (University of Aberdeen), for lab management and technical support, the British Heart Foundation (PG/13/23/30080) for research funding, and the Institute of Medical Sciences (University of Aberdeen) for PhD studentship funding.


  • mouse embryonic stem cell
  • cardiomyocytes
  • heart
  • in vitro differentiation


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