Abstract
A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.
Original language | English |
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Pages (from-to) | 345-52 |
Number of pages | 8 |
Journal | Plant Molecular Biology |
Volume | 34 |
Issue number | 2 |
Publication status | Published - 1 May 1997 |
Keywords
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
- DNA, Plant
- Gene Expression Regulation, Developmental
- Genes, Plant
- HSP70 Heat-Shock Proteins
- Molecular Sequence Data
- Peas
- Plant Proteins
- RNA, Messenger
- Sequence Analysis, DNA