Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss

Zhitao Qi; Qihuan Zhang; Jason W. Holland; Qian Gao; Carolina Tafalla; Xiuchun Wang; Tiehui Wang

doi:10.1007/s12562-016-0997-5

Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss

Zhitao Qi^*, Qihuan Zhang, Jason W. Holland, Qian Gao, Carolina Tafalla, Xiuchun Wang, Tiehui Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM⁺ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.

Original language	English
Pages (from-to)	613-622
Number of pages	10
Journal	Reviews in Fisheries Science
Volume	82
Issue number	4
Early online date	20 Jun 2016
DOIs	https://doi.org/10.1007/s12562-016-0997-5
Publication status	Published - 1 Jul 2016

Bibliographical note

Acknowledgments
This work was supported by the Scottish Funding Council for the MASTS (the Marine Alliance for Science and Technology for Scotland) (grant reference HR09011) and partially by grants from the National Natural Science Foundation of China (31302221, 31272666, and 31470130). ZQ was financially supported by the “Qinglan” project of Jiangsu Province and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. We thank Professor Mingxian Chang, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, for her kind comments on the phylogenetic tree analysis.

Keywords

Chemokine-like receptors
CMKLR3
Expression
Oncorhynchus mykiss
Rainbow trout

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title = "Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss",

abstract = "Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.",

keywords = "Chemokine-like receptors, CMKLR3, Expression, Oncorhynchus mykiss, Rainbow trout",

author = "Zhitao Qi and Qihuan Zhang and Holland, {Jason W.} and Qian Gao and Carolina Tafalla and Xiuchun Wang and Tiehui Wang",

note = "Acknowledgments This work was supported by the Scottish Funding Council for the MASTS (the Marine Alliance for Science and Technology for Scotland) (grant reference HR09011) and partially by grants from the National Natural Science Foundation of China (31302221, 31272666, and 31470130). ZQ was financially supported by the “Qinglan” project of Jiangsu Province and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. We thank Professor Mingxian Chang, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, for her kind comments on the phylogenetic tree analysis.",

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AU - Tafalla, Carolina

AU - Wang, Xiuchun

AU - Wang, Tiehui

N1 - Acknowledgments This work was supported by the Scottish Funding Council for the MASTS (the Marine Alliance for Science and Technology for Scotland) (grant reference HR09011) and partially by grants from the National Natural Science Foundation of China (31302221, 31272666, and 31470130). ZQ was financially supported by the “Qinglan” project of Jiangsu Province and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. We thank Professor Mingxian Chang, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, for her kind comments on the phylogenetic tree analysis.

PY - 2016/7/1

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N2 - Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.

AB - Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.

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SN - 0919-9268

VL - 82

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EP - 622

JO - Reviews in Fisheries Science

JF - Reviews in Fisheries Science

IS - 4

ER -

Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss

Abstract

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Keywords

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