Abstract
This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals.
Original language | English |
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Pages (from-to) | 505-516 |
Number of pages | 12 |
Journal | Developmental and Comparative Immunology |
Volume | 38 |
Issue number | 4 |
Early online date | 20 Sept 2012 |
DOIs | |
Publication status | Published - Dec 2012 |
Bibliographical note
Copyright © 2012 Elsevier Ltd. All rights reserved.Keywords
- aeromonas salmonicida
- amino acid sequence
- animals
- aquaculture
- base sequence
- cloning, molecular
- fish diseases
- fish proteins
- flatfishes
- gene expression
- gram-negative bacterial infections
- head kidney
- interleukin-17
- interleukins
- liver
- models, molecular
- molecular sequence data
- organ specificity
- phylogeny
- protein structure, tertiary
- sequence analysis, DNA
- spleen
- structural homology, protein
- Th17 cells