Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus

M M Costa, P Pereiro, T Wang, C J Secombes, A Figueras, B Novoa, Tiehui Wang

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35 Citations (Scopus)


This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals.
Original languageEnglish
Pages (from-to)505-516
Number of pages12
JournalDevelopmental and Comparative Immunology
Issue number4
Early online date20 Sept 2012
Publication statusPublished - Dec 2012

Bibliographical note

Copyright © 2012 Elsevier Ltd. All rights reserved.


  • aeromonas salmonicida
  • amino acid sequence
  • animals
  • aquaculture
  • base sequence
  • cloning, molecular
  • fish diseases
  • fish proteins
  • flatfishes
  • gene expression
  • gram-negative bacterial infections
  • head kidney
  • interleukin-17
  • interleukins
  • liver
  • models, molecular
  • molecular sequence data
  • organ specificity
  • phylogeny
  • protein structure, tertiary
  • sequence analysis, DNA
  • spleen
  • structural homology, protein
  • Th17 cells


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