The finding of the enigmatic pathogen Anisolpidium rosenvingei in the filamentous brown macroalga Pylaiella littoralis presented a unique opportunity to histochemically study the distribution of chitin in this little-known pathogen using FUNGALASE™-F, a fluorescein-labelled chitinase. Chitin was found localised to the exit tube of this pathogen, which infects exclusively reproductive cells of its host. The cytological and phylogenetic implications of this finding are discussed. This paper also reports the first record of this pathogen in the United Kingdom, on the west coast of Scotland.
We acknowledge the support of the European Community research infrastructure action under the FP7 ‘capacities’ specific program ASSEMBLE (grant no. 227788) for supporting travel of FCK and KIF to Roscoff in 2013. We would also like to thank the TOTAL Foundation (Paris) and the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) - their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. Finally we would like to thank the Natural Environment Research Council (NERC) for funding Kyle Fletcher’s PhD studentship.