Abstract
CLEC-2 is a member of the "dectin-1 cluster" of C-type lectin-like receptors and was originally thought to be restricted to platelets. In this study, we demonstrate that murine CLEC-2 is also expressed by peripheral blood neutrophils, but only weakly by bone marrow or elicited inflammatory neutrophils. On circulating neutrophils, CLEC-2 can mediate phagocytosis of Ab-coated beads and the production of proinflammatory cytokines, including TNF-alpha, in response to the CLEC-2 ligand, rhodocytin. CLEC-2 possesses a tyrosine-based cytoplasmic motif similar to that of dectin-1, and we show using chimeric analyses that the activities of this receptor are dependent on this tyrosine. Like dectin-1, CLEC-2 can recruit the signaling kinase Syk in myeloid cells, however, stimulation of this pathway does not induce the respiratory burst. These data therefore demonstrate that CLEC-2 expression is not restricted to platelets and that it functions as an activation receptor on neutrophils.
Original language | English |
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Pages (from-to) | 4150-4157 |
Number of pages | 8 |
Journal | The Journal of Immunology |
Volume | 182 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1 Apr 2009 |
Keywords
- animals
- blotting, western
- enzyme-linked immunosorbent assay
- flow cytometry
- gene expression
- gene expression regulation
- immunoprecipitation
- intracellular signaling peptides and proteins
- lectins, C-type
- mice
- neutrophils
- phagocytosis
- protein-tyrosine kinases
- respiratory burst
- tumor necrosis factor-alpha