Clonal Strain Persistence of Candida albicans Isolates from Chronic Mucocutaneous Candidiasis Patients

Alexander J. Moorhouse, Claire Rennison, Muhammad Raza, Desa Lilic, Neil A. R. Gow

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19 Citations (Scopus)
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Chronic mucocutaneous candidiasis (CMC) is a primary immunodeficiency disorder characterised by susceptibility to chronic Candida and fungal dermatophyte infections of the skin, nails and mucous membranes. Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced. We subjected 42 C. albicans individual single colony isolates from 6 unrelated CMC patients to multilocus sequence typing (MLST). Multiple colonies were typed from swabs taken from multiple body sites across multiple time points over a 17-month period. Among isolates from each individual patient, our data show clonal and persistent diploid sequence types (DSTs) that were stable over time, identical between multiple infection sites and exhibit azole resistant phenotypes. No shared origin or common source of infection was identified among isolates from these patients. Additionally, we performed C. albicans MLST SNP genotype frequency analysis to identify signatures of past loss of heterozygosity (LOH) events among persistent and azole resistant isolates retrieved from patients with autoimmune disorders including CMC.
Original languageEnglish
Article number0145888
Pages (from-to)1-15
Number of pages15
JournalPloS ONE
Issue number2
Publication statusPublished - 5 Feb 2016

Bibliographical note

Funding: The study was supported by the MRC for a PhD studentship for AM and the Wellcome Trust for funding (086827, 075470, 097377 & 101873).

We thank Professor Frank Odds for MLST central database curation and useful discussions.


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