Deciphering the Immunostimulatory Effects of β-Glucan on a Rainbow Trout (Oncorhynchus mykiss) Macrophage-like Cell Line (RTS11) by Whole Transcriptome Analysis: Genes

Dean Porter; Shahmir Naseer; David Peggs; Charles McGurk; Samuel A. Martin

doi:10.3390/genes14061261

Deciphering the Immunostimulatory Effects of β-Glucan on a Rainbow Trout (Oncorhynchus mykiss) Macrophage-like Cell Line (RTS11) by Whole Transcriptome Analysis: Genes

Dean Porter, Shahmir Naseer, David Peggs, Charles McGurk, Samuel A. Martin^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

β-glucans are a commonly used immunostimulant/prebiotic in many aquaculture applications for boosting the immune status in fish. However, the method of action as an immunostimulant has not been fully deciphered. To determine the immunomodulatory effects of β-glucans on the innate immune response, we stimulated the rainbow trout spleen macrophage-like cell line (RTS11) with β-1,3/1,6-glucans for 4 h. This study uses a whole transcriptomic approach to analyse the immunomodulatory properties of β-glucans. Several proinflammatory pathways were found to be enriched after stimulation, demonstrating the immunomodulatory effects of β-glucan supplementation. Several pathways relating to responses to bacteria were also found to be enriched. This study clearly demonstrates the immunomodulatory effects of the supplementation of β-glucans within an aquaculture setting and further validates the use of cell lines as predictive models to interpret the responses caused by dietary intervention.

Original language	English
Article number	1261
Number of pages	17
Journal	Genes
Volume	14
Issue number	6
DOIs	https://doi.org/10.3390/genes14061261
Publication status	Published - 14 Jun 2023

Bibliographical note

Acknowledgments
The technical support provided by Dawn Shewring was greatly appreciated. The RTS11 cells were donated as a gift from Niels Bols to researchers at the Scottish Fish Immunology Research Centre, University of Aberdeen.

Funding
This work was funded by the University of Aberdeen and Skretting AI.

Data Availability Statement

The RNA-seq data have been deposited in the ArrayExpress repository (http://www.ebi.ac.uk/arrayexpress/) under accession number E-MTAB-12643.

Keywords

RTS11
RNA-seq
transcriptomics
β-glucan
immunomodulation
innate immune response

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Porter_Etal_G_Deciphering_The_Immunostimulatory_VOR
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Final published version, 2.03 MBLicence: CC BY

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title = "Deciphering the Immunostimulatory Effects of β-Glucan on a Rainbow Trout (Oncorhynchus mykiss) Macrophage-like Cell Line (RTS11) by Whole Transcriptome Analysis: Genes",

abstract = "β-glucans are a commonly used immunostimulant/prebiotic in many aquaculture applications for boosting the immune status in fish. However, the method of action as an immunostimulant has not been fully deciphered. To determine the immunomodulatory effects of β-glucans on the innate immune response, we stimulated the rainbow trout spleen macrophage-like cell line (RTS11) with β-1,3/1,6-glucans for 4 h. This study uses a whole transcriptomic approach to analyse the immunomodulatory properties of β-glucans. Several proinflammatory pathways were found to be enriched after stimulation, demonstrating the immunomodulatory effects of β-glucan supplementation. Several pathways relating to responses to bacteria were also found to be enriched. This study clearly demonstrates the immunomodulatory effects of the supplementation of β-glucans within an aquaculture setting and further validates the use of cell lines as predictive models to interpret the responses caused by dietary intervention.",

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author = "Dean Porter and Shahmir Naseer and David Peggs and Charles McGurk and Martin, {Samuel A.}",

note = "Acknowledgments The technical support provided by Dawn Shewring was greatly appreciated. The RTS11 cells were donated as a gift from Niels Bols to researchers at the Scottish Fish Immunology Research Centre, University of Aberdeen. Funding This work was funded by the University of Aberdeen and Skretting AI.",

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N1 - Acknowledgments The technical support provided by Dawn Shewring was greatly appreciated. The RTS11 cells were donated as a gift from Niels Bols to researchers at the Scottish Fish Immunology Research Centre, University of Aberdeen. Funding This work was funded by the University of Aberdeen and Skretting AI.

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N2 - β-glucans are a commonly used immunostimulant/prebiotic in many aquaculture applications for boosting the immune status in fish. However, the method of action as an immunostimulant has not been fully deciphered. To determine the immunomodulatory effects of β-glucans on the innate immune response, we stimulated the rainbow trout spleen macrophage-like cell line (RTS11) with β-1,3/1,6-glucans for 4 h. This study uses a whole transcriptomic approach to analyse the immunomodulatory properties of β-glucans. Several proinflammatory pathways were found to be enriched after stimulation, demonstrating the immunomodulatory effects of β-glucan supplementation. Several pathways relating to responses to bacteria were also found to be enriched. This study clearly demonstrates the immunomodulatory effects of the supplementation of β-glucans within an aquaculture setting and further validates the use of cell lines as predictive models to interpret the responses caused by dietary intervention.

AB - β-glucans are a commonly used immunostimulant/prebiotic in many aquaculture applications for boosting the immune status in fish. However, the method of action as an immunostimulant has not been fully deciphered. To determine the immunomodulatory effects of β-glucans on the innate immune response, we stimulated the rainbow trout spleen macrophage-like cell line (RTS11) with β-1,3/1,6-glucans for 4 h. This study uses a whole transcriptomic approach to analyse the immunomodulatory properties of β-glucans. Several proinflammatory pathways were found to be enriched after stimulation, demonstrating the immunomodulatory effects of β-glucan supplementation. Several pathways relating to responses to bacteria were also found to be enriched. This study clearly demonstrates the immunomodulatory effects of the supplementation of β-glucans within an aquaculture setting and further validates the use of cell lines as predictive models to interpret the responses caused by dietary intervention.

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ER -

Deciphering the Immunostimulatory Effects of β-Glucan on a Rainbow Trout (Oncorhynchus mykiss) Macrophage-like Cell Line (RTS11) by Whole Transcriptome Analysis: Genes

Abstract

Bibliographical note

Data Availability Statement

Keywords

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