Description of Ruminococcoides bili gen. nov., sp. nov., a bile resistant bacterium from human bile with autolytic behavior.

Natalia Molinero , Elena Conti, Borja Sánchez, Alan W. Walker, Abelardo Margolles, Sylvia H. Duncan, Susana Delgado * (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

A strictly anaerobic, resistant starch-degrading, bile-tolerant, autolytic strain, IPLA60002T, belonging to the family Ruminococcaceae , was isolated from a human bile sample of a liver donor without hepatobiliary disease. Cells were Gram-stain-positive cocci, and 16S rRNA gene and whole genome analyses showed that Ruminococcus bromii was the phylogenetically closest related species to the novel strain IPLA60002T, though with average nucleotide identity values below 90 %. Biochemically, the new isolate has metabolic features similar to those described previously for gut R. bromii strains, including the ability to degrade a range of different starches. The new isolate, however, produces lactate and shows distinct resistance to the presence of bile salts. Additionally, the novel bile isolate displays an autolytic phenotype after growing in different media. Strain IPLA60002T is phylogenetically distinct from other species within the genus Ruminococcus . Therefore, we propose on the basis of phylogenetic, genomic and metabolic data that the novel IPLA60002T strain isolated from human bile be given the name Ruminococcoides bili gen. nov., sp. nov., within the new proposed genus Ruminococcoides and the family Ruminococcaceae . Strain IPLA60002T (=DSM 110008T=LMG 31505T) is proposed as the type strain of Ruminococcoides bili.
Original languageEnglish
Article number004960
Number of pages11
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume71
Issue number8
Early online date16 Aug 2021
DOIs
Publication statusPublished - Aug 2021

Bibliographical note

Funding: This study was supported by

Tenovus (GB) - Principle Award Recipient: Elena Conti
Rural and Environment Science and Analytical Services Division (GB) (Award SG-RESAS) - Principle Award Recipient: Sylvia H. Duncan
Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología (Award IDI/2018/000236)
Ministerio de Economía y Competitividad (Award Ramón y Cajal” program (RYC-2016-19726)) - Principle Award Recipient: Susana Delgado
Ministerio de Economía y Competitividad (Award FPI Predoctoral Grant BES-2014-068736) - Principle Award Recipient: Natalia Molinero
Ministerio de Economía y Competitividad (Award AGL2013-44761-P) - Principle Award Recipient: Abelardo Margolles

Data Availability Statement

Four supplementary figures are available with the online version of this article.

Keywords

  • autolysis
  • bile resistance
  • human bile isolate
  • Ruminococcoides

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