Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium legunninosarum biovar trifolii

G. I. Paton; G. Palmer; M. Burton; E. A.S. Rattray; S. P. McGrath; L. A. Glover; K. Killham

doi:10.1046/j.1472-765X.1997.00071.x

Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium legunninosarum biovar trifolii

G. I. Paton^*, G. Palmer, M. Burton, E. A.S. Rattray, S. P. McGrath, L. A. Glover, K. Killham

^*Corresponding author for this work

Rothamsted Research

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

A soil isolate of Rhizobium leguminosarum by trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminiscence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.

Original language	English
Pages (from-to)	296-300
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	24
Issue number	4
DOIs	https://doi.org/10.1046/j.1472-765X.1997.00071.x
Publication status	Published - 1 Jan 1997

Access to Document

10.1046/j.1472-765X.1997.00071.x

Cite this

@article{96b9e07887214832ad73238862af7069,

title = "Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium legunninosarum biovar trifolii",

abstract = "A soil isolate of Rhizobium leguminosarum by trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminiscence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.",

author = "Paton, {G. I.} and G. Palmer and M. Burton and Rattray, {E. A.S.} and McGrath, {S. P.} and Glover, {L. A.} and K. Killham",

year = "1997",

month = jan,

day = "1",

doi = "10.1046/j.1472-765X.1997.00071.x",

language = "English",

volume = "24",

pages = "296--300",

journal = "Letters in Applied Microbiology",

issn = "0266-8254",

publisher = "Oxford University Press (OUP)",

number = "4",

}

TY - JOUR

T1 - Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium legunninosarum biovar trifolii

AU - Paton, G. I.

AU - Palmer, G.

AU - Burton, M.

AU - Rattray, E. A.S.

AU - McGrath, S. P.

AU - Glover, L. A.

AU - Killham, K.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - A soil isolate of Rhizobium leguminosarum by trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminiscence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.

AB - A soil isolate of Rhizobium leguminosarum by trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminiscence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.

UR - http://www.scopus.com/inward/record.url?scp=0030613314&partnerID=8YFLogxK

U2 - 10.1046/j.1472-765X.1997.00071.x

DO - 10.1046/j.1472-765X.1997.00071.x

M3 - Article

C2 - 9134778

AN - SCOPUS:0030613314

SN - 0266-8254

VL - 24

SP - 296

EP - 300

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 4

ER -

Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium legunninosarum biovar trifolii

Abstract

Access to Document

Other files and links

Fingerprint

Cite this