Development of eukaryotic zoospores within polycyclic aromatic hydrocarbon (PAH)-polluted environments: A set of behaviors that are relevant for bioremediation

Rungroch  Sungthong; Pieter Van West; Manuel Cantos; Jose Julio  Ortega-Calvo

doi:10.1016/j.scitotenv.2014.12.089

Development of eukaryotic zoospores within polycyclic aromatic hydrocarbon (PAH)-polluted environments: A set of behaviors that are relevant for bioremediation

Rungroch Sungthong, Pieter Van West, Manuel Cantos, Jose Julio Ortega-Calvo

Instituto de Recursos Naturales y Agrobiologica de Sevilla

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

In this study, we assessed the development (formation, taxis and settlement) of eukaryotic zoospores under different regimes of exposure to polycyclic aromatic hydrocarbons (PAHs), which imitated environmental scenarios of pollution and bioremediation. With this aim, we used an oomycete, Pythium aphanidermatum, as a source of zoospores and two PAH-degrading bacteria (Mycobacterium gilvum VM552 and Pseudomonas putida G7). The oomycete and both bacteria were not antagonistic, and zoospore formation was diminished only in the presence of the highest bacterial cell density (108–1010 colony-forming units mL− 1). A negative influence of PAHs on zoospore formation and taxis was observed when PAHs were exposed in combination with organic solutions and polar solvents. Co-exposure of PAHs with non-polar solvents [hexadecane (HD) and 2,2,4,4,6,8,8-heptamethylnonane (HMN)] did not affect zoospore settlement at the interfaces of the organic solvents and water. However, zoospores settled and created mycelial networks only at HD–water interfaces. Both bacteria diminished the toxic influence of PAHs on zoospore formation and taxis, and they did not interrupt zoospore settlement. The results suggest that zoospore development could be applicable for toxicity assessment of PAHs and enhancement of their bioavailability. Microbial interactions during both swimming modes and community formation at pollutant interfaces were revealed as major factors that have potential relevance to bioremediation.

Original language	English
Pages (from-to)	767-776
Number of pages	10
Journal	Science of the Total Environment
Volume	511
Early online date	22 Jan 2015
DOIs	https://doi.org/10.1016/j.scitotenv.2014.12.089
Publication status	Published - 1 Apr 2015

Bibliographical note

Support for this research was provided by the Spanish Ministry of Science and Innovation (CGL2010-22068-C02-01 and CGL2013-44554-R), the Andalusian Government (RNM 2337) and the CSIC JAE Programme (RS). PvW has funding support from the BBSRC and NERC. We are grateful to Dr. Herbert van den Berg (Aberdeen Oomycete Laboratory, University of Aberdeen) for preparing the initial stock of Py. aphanidermatum and delivering it to us and Dr. Fredrik Heyman (Uppsala BioCenter, Swedish University of Agricultural Sciences) for his guidance regarding production of oomycete zoospores.

Keywords

eukaryotic zoospore
oomycetes
PAH-polluted scenario
PAH-degrading bacteria
zoospore development
bioremediation

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title = "Development of eukaryotic zoospores within polycyclic aromatic hydrocarbon (PAH)-polluted environments: A set of behaviors that are relevant for bioremediation",

abstract = "In this study, we assessed the development (formation, taxis and settlement) of eukaryotic zoospores under different regimes of exposure to polycyclic aromatic hydrocarbons (PAHs), which imitated environmental scenarios of pollution and bioremediation. With this aim, we used an oomycete, Pythium aphanidermatum, as a source of zoospores and two PAH-degrading bacteria (Mycobacterium gilvum VM552 and Pseudomonas putida G7). The oomycete and both bacteria were not antagonistic, and zoospore formation was diminished only in the presence of the highest bacterial cell density (108–1010 colony-forming units mL− 1). A negative influence of PAHs on zoospore formation and taxis was observed when PAHs were exposed in combination with organic solutions and polar solvents. Co-exposure of PAHs with non-polar solvents [hexadecane (HD) and 2,2,4,4,6,8,8-heptamethylnonane (HMN)] did not affect zoospore settlement at the interfaces of the organic solvents and water. However, zoospores settled and created mycelial networks only at HD–water interfaces. Both bacteria diminished the toxic influence of PAHs on zoospore formation and taxis, and they did not interrupt zoospore settlement. The results suggest that zoospore development could be applicable for toxicity assessment of PAHs and enhancement of their bioavailability. Microbial interactions during both swimming modes and community formation at pollutant interfaces were revealed as major factors that have potential relevance to bioremediation.",

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author = "Rungroch Sungthong and {Van West}, Pieter and Manuel Cantos and Ortega-Calvo, {Jose Julio}",

note = "Support for this research was provided by the Spanish Ministry of Science and Innovation (CGL2010-22068-C02-01 and CGL2013-44554-R), the Andalusian Government (RNM 2337) and the CSIC JAE Programme (RS). PvW has funding support from the BBSRC and NERC. We are grateful to Dr. Herbert van den Berg (Aberdeen Oomycete Laboratory, University of Aberdeen) for preparing the initial stock of Py. aphanidermatum and delivering it to us and Dr. Fredrik Heyman (Uppsala BioCenter, Swedish University of Agricultural Sciences) for his guidance regarding production of oomycete zoospores.",

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AU - Ortega-Calvo, Jose Julio

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KW - eukaryotic zoospore

KW - oomycetes

KW - PAH-polluted scenario

KW - PAH-degrading bacteria

KW - zoospore development

KW - bioremediation

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DO - 10.1016/j.scitotenv.2014.12.089

M3 - Article

SN - 0048-9697

VL - 511

SP - 767

EP - 776

JO - Science of the Total Environment

JF - Science of the Total Environment

ER -

Development of eukaryotic zoospores within polycyclic aromatic hydrocarbon (PAH)-polluted environments: A set of behaviors that are relevant for bioremediation

Abstract

Bibliographical note

Keywords

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