A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.
|Number of pages||4|
|Journal||Analytical and Bioanalytical Chemistry|
|Publication status||Published - Jul 2004|
|Event||3rd International Conference on Instrumental Methods of Analysis - Thessaloniki, Greece|
Duration: 23 Sept 2003 → 27 Sept 2003
Bibliographical noteFrom the issue entitled "Instrumental Methods of Analysis"
- exchangeable copper
- atomic absorption spectrometry
- blood plasma