Dialysis-Chelex method for determination of exchangeable copper in human plasma

Tony I. Venelinov; I. M. Davies; John Hamilton Beattie

doi:10.1007/s00216-004-2529-x

Dialysis-Chelex method for determination of exchangeable copper in human plasma

Tony I. Venelinov, I. M. Davies, John Hamilton Beattie

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.

Original language	English
Pages (from-to)	777-780
Number of pages	4
Journal	Analytical and Bioanalytical Chemistry
Volume	379
Issue number	5-6
DOIs	https://doi.org/10.1007/s00216-004-2529-x
Publication status	Published - Jul 2004
Event	3rd International Conference on Instrumental Methods of Analysis - Thessaloniki, Greece Duration: 23 Sept 2003 → 27 Sept 2003

Bibliographical note

From the issue entitled "Instrumental Methods of Analysis"

Keywords

exchangeable copper
dialysis
Chelex-100
ICP-MS
atomic absorption spectrometry
blood plasma
serum
zinc
speciation
binding

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10.1007/s00216-004-2529-x

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title = "Dialysis-Chelex method for determination of exchangeable copper in human plasma",

abstract = "A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.",

keywords = "exchangeable copper, dialysis, Chelex-100, ICP-MS, atomic absorption spectrometry, blood plasma, serum, zinc, speciation, binding",

author = "Venelinov, {Tony I.} and Davies, {I. M.} and Beattie, {John Hamilton}",

note = "From the issue entitled {"}Instrumental Methods of Analysis{"}; 3rd International Conference on Instrumental Methods of Analysis ; Conference date: 23-09-2003 Through 27-09-2003",

year = "2004",

month = jul,

doi = "10.1007/s00216-004-2529-x",

language = "English",

volume = "379",

pages = "777--780",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer Verlag",

number = "5-6",

}

TY - JOUR

T1 - Dialysis-Chelex method for determination of exchangeable copper in human plasma

AU - Venelinov, Tony I.

AU - Davies, I. M.

AU - Beattie, John Hamilton

N1 - From the issue entitled "Instrumental Methods of Analysis"

PY - 2004/7

Y1 - 2004/7

N2 - A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.

AB - A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.

KW - exchangeable copper

KW - dialysis

KW - Chelex-100

KW - ICP-MS

KW - atomic absorption spectrometry

KW - blood plasma

KW - serum

KW - zinc

KW - speciation

KW - binding

U2 - 10.1007/s00216-004-2529-x

DO - 10.1007/s00216-004-2529-x

M3 - Article

SN - 1618-2642

VL - 379

SP - 777

EP - 780

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 5-6

T2 - 3rd International Conference on Instrumental Methods of Analysis

Y2 - 23 September 2003 through 27 September 2003

ER -

Dialysis-Chelex method for determination of exchangeable copper in human plasma

Abstract

Bibliographical note

Keywords

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