Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events

K I Shennan; N A Taylor; J L Jermany; G Matthews; K Docherty

Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events

K I Shennan, N A Taylor, J L Jermany, G Matthews, K Docherty

Research output: Contribution to journal › Article › peer-review

Abstract

PC2 and PC3, which is also known as PC1, are subtilisin-like proteases that are involved in the intracellular processing of prohormones and proneuropeptides. Both enzymes are synthesized as propolypeptides that undergo proteolytic maturation within the secretory pathway. An in vitro translation/translocation system from Xenopus egg extracts was used to investigate mechanisms in the maturation of pro-PC3 and pro-PC2. Pro-PC3 underwent rapid (t1/2

Original language	English
Pages (from-to)	1402-7
Number of pages	6
Journal	The Journal of Biological Chemistry
Volume	270
Issue number	3
Publication status	Published - 20 Jan 1995

Keywords

Amino Acid Sequence
Animals
Aspartic Acid Endopeptidases
Base Sequence
Biological Transport
Calcium
Hydrogen-Ion Concentration
Molecular Sequence Data
Oligodeoxyribonucleotides
Proprotein Convertase 2
Proprotein Convertases
Protein Processing, Post-Translational
RNA, Messenger
Subtilisins
Xenopus

Cite this

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title = "Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events",

abstract = "PC2 and PC3, which is also known as PC1, are subtilisin-like proteases that are involved in the intracellular processing of prohormones and proneuropeptides. Both enzymes are synthesized as propolypeptides that undergo proteolytic maturation within the secretory pathway. An in vitro translation/translocation system from Xenopus egg extracts was used to investigate mechanisms in the maturation of pro-PC3 and pro-PC2. Pro-PC3 underwent rapid (t1/2 ",

keywords = "Amino Acid Sequence, Animals, Aspartic Acid Endopeptidases, Base Sequence, Biological Transport, Calcium, Hydrogen-Ion Concentration, Molecular Sequence Data, Oligodeoxyribonucleotides, Proprotein Convertase 2, Proprotein Convertases, Protein Processing, Post-Translational, RNA, Messenger, Subtilisins, Xenopus",

author = "Shennan, {K I} and Taylor, {N A} and Jermany, {J L} and G Matthews and K Docherty",

year = "1995",

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language = "English",

volume = "270",

pages = "1402--7",

journal = "The Journal of Biological Chemistry",

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TY - JOUR

T1 - Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events

AU - Shennan, K I

AU - Taylor, N A

AU - Jermany, J L

AU - Matthews, G

AU - Docherty, K

PY - 1995/1/20

Y1 - 1995/1/20

N2 - PC2 and PC3, which is also known as PC1, are subtilisin-like proteases that are involved in the intracellular processing of prohormones and proneuropeptides. Both enzymes are synthesized as propolypeptides that undergo proteolytic maturation within the secretory pathway. An in vitro translation/translocation system from Xenopus egg extracts was used to investigate mechanisms in the maturation of pro-PC3 and pro-PC2. Pro-PC3 underwent rapid (t1/2

AB - PC2 and PC3, which is also known as PC1, are subtilisin-like proteases that are involved in the intracellular processing of prohormones and proneuropeptides. Both enzymes are synthesized as propolypeptides that undergo proteolytic maturation within the secretory pathway. An in vitro translation/translocation system from Xenopus egg extracts was used to investigate mechanisms in the maturation of pro-PC3 and pro-PC2. Pro-PC3 underwent rapid (t1/2

KW - Amino Acid Sequence

KW - Animals

KW - Aspartic Acid Endopeptidases

KW - Base Sequence

KW - Biological Transport

KW - Calcium

KW - Hydrogen-Ion Concentration

KW - Molecular Sequence Data

KW - Oligodeoxyribonucleotides

KW - Proprotein Convertase 2

KW - Proprotein Convertases

KW - Protein Processing, Post-Translational

KW - RNA, Messenger

KW - Subtilisins

KW - Xenopus

M3 - Article

C2 - 7836407

SN - 0021-9258

VL - 270

SP - 1402

EP - 1407

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

IS - 3

ER -

Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events

Abstract

Keywords

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