Different distributions of dystrophin and related proteins at nerve-muscle junctions

Guy Smith Bewick, L V Nicholson, C Young, E O'Donnell, C R Slater

Research output: Contribution to journalArticlepeer-review

127 Citations (Scopus)


The distributions of dystrophin, 'dystrophin-related protein' (DRP) and beta-spectrin were compared with that of acetylcholine receptors (AChRs) at rat nerve-muscle junctions (NMJs) using immunofluorescence techniques. In sections, monoclonal antibodies (MAbs) to dystrophin and beta-spectrin labelled the entire sarcolemma but were concentrated at the NMJs while those to DRP labelled only NMJs. In permeabilized muscle fibres, DRP was precisely co-localized with the AChRs, whereas the zone of high density labelling of dystrophin and beta-spectrin extended 0.3-0.4 microns beyond the AChRs. Within the NMJ, the labelling of DRP appeared as a series of interconnecting lines similar to that of AChRs. However, labelling of dystrophin and beta-spectrin was consistently more punctate. These data suggest DRP is more closely associated with AChRs than are dystrophin or beta-spectrin.
Original languageEnglish
Pages (from-to)857-860
Number of pages4
Issue number10
Publication statusPublished - 1 Oct 1992


  • Animals
  • Antibodies, Monoclonal
  • Cytoskeleton
  • Dystrophin
  • Female
  • Immunohistochemistry
  • Neuromuscular Junction
  • Rats
  • Rats, Wistar
  • Receptors, Cholinergic


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