Different Candida parapsilosis clinical isolates and lipase deficient strain trigger an altered cellular immune response

Renáta Tóth; Maria F Alonso; Judith M Bain; Csaba Vágvölgyi; Lars-Peter Erwig; Attila Gácser

doi:10.3389/fmicb.2015.01102

Different Candida parapsilosis clinical isolates and lipase deficient strain trigger an altered cellular immune response

Renáta Tóth, Maria F Alonso, Judith M Bain, Csaba Vágvölgyi, Lars-Peter Erwig, Attila Gácser

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Abstract

Numerous human diseases can be associated with fungal infections either as potential causative agents or as a result of changed immune status due to a primary disease. Fungal infections caused by Candida species can vary from mild to severe dependent upon the site of infection, length of exposure, and past medical history. Patients with impaired immune status are at increased risk for chronic fungal infections. Recent epidemiologic studies have revealed the increasing incidence of candidiasis caused by non-albicans species such as Candida parapsilosis. Due to its increasing relevance we chose two distinct C. parapsilosis strains, to describe the cellular innate immune response toward this species. In the first section of our study we compared the interaction of CLIB 214 and GA1 cells with murine and human macrophages. Both strains are commonly used to investigate C. parapsilosis virulence properties. CLIB 214 is a rapidly pseudohyphae-forming strain and GA1 is an isolate that mainly exists in a yeast form. Our results showed, that the phagocyte response was similar in terms of overall uptake, however differences were observed in macrophage migration and engulfment of fungal cells. As C. parapsilosis releases extracellular lipases in order to promote host invasion we further investigated the role of these secreted components during the distinct stages of the phagocytic process. Using a secreted lipase deficient mutant strain and the parental strain GA1 individually and simultaneously, we confirmed that fungal secreted lipases influence the fungi's virulence by detecting altered innate cellular responses. In this study we report that two isolates of a single species can trigger markedly distinct host responses and that lipase secretion plays a role on the cellular level of host-pathogen interactions.

Original language	English
Article number	1102
Number of pages	11
Journal	Frontiers in Microbiology
Volume	6
Early online date	13 Oct 2015
DOIs	https://doi.org/10.3389/fmicb.2015.01102
Publication status	Published - 13 Oct 2015

Bibliographical note

Funding
RT's research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4. A/2-11-1-2012-0001 “National Excellence Program.” AG is supported by OTKA NN113153 and by NF84006. AG is further supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. This work was supported by the Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377 to LE. CV thanks the Visiting Professor Program, Deanship of Scientific Research at King Saud University, Riyadh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

Candida parapsilosis
phagocyte response
secreted lipase
co-infection
live cell imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Different Candida parapsilosis clinical isolates and lipase deficient strain trigger an altered cellular immune response
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/
Final published version, 2.79 MBLicence: CC BY

Cite this

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title = "Different Candida parapsilosis clinical isolates and lipase deficient strain trigger an altered cellular immune response",

abstract = "Numerous human diseases can be associated with fungal infections either as potential causative agents or as a result of changed immune status due to a primary disease. Fungal infections caused by Candida species can vary from mild to severe dependent upon the site of infection, length of exposure, and past medical history. Patients with impaired immune status are at increased risk for chronic fungal infections. Recent epidemiologic studies have revealed the increasing incidence of candidiasis caused by non-albicans species such as Candida parapsilosis. Due to its increasing relevance we chose two distinct C. parapsilosis strains, to describe the cellular innate immune response toward this species. In the first section of our study we compared the interaction of CLIB 214 and GA1 cells with murine and human macrophages. Both strains are commonly used to investigate C. parapsilosis virulence properties. CLIB 214 is a rapidly pseudohyphae-forming strain and GA1 is an isolate that mainly exists in a yeast form. Our results showed, that the phagocyte response was similar in terms of overall uptake, however differences were observed in macrophage migration and engulfment of fungal cells. As C. parapsilosis releases extracellular lipases in order to promote host invasion we further investigated the role of these secreted components during the distinct stages of the phagocytic process. Using a secreted lipase deficient mutant strain and the parental strain GA1 individually and simultaneously, we confirmed that fungal secreted lipases influence the fungi's virulence by detecting altered innate cellular responses. In this study we report that two isolates of a single species can trigger markedly distinct host responses and that lipase secretion plays a role on the cellular level of host-pathogen interactions.",

keywords = "Candida parapsilosis, phagocyte response, secreted lipase, co-infection, live cell imaging",

author = "Ren{\'a}ta T{\'o}th and Alonso, {Maria F} and Bain, {Judith M} and Csaba V{\'a}gv{\"o}lgyi and Lars-Peter Erwig and Attila G{\'a}cser",

note = "Funding RT's research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T{\'A}MOP 4.2.4. A/2-11-1-2012-0001 “National Excellence Program.” AG is supported by OTKA NN113153 and by NF84006. AG is further supported by the J{\'a}nos Bolyai Research Scholarship of the Hungarian Academy of Sciences. This work was supported by the Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377 to LE. CV thanks the Visiting Professor Program, Deanship of Scientific Research at King Saud University, Riyadh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. ",

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AU - Tóth, Renáta

AU - Alonso, Maria F

AU - Bain, Judith M

AU - Vágvölgyi, Csaba

AU - Erwig, Lars-Peter

AU - Gácser, Attila

N1 - Funding RT's research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4. A/2-11-1-2012-0001 “National Excellence Program.” AG is supported by OTKA NN113153 and by NF84006. AG is further supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. This work was supported by the Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377 to LE. CV thanks the Visiting Professor Program, Deanship of Scientific Research at King Saud University, Riyadh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PY - 2015/10/13

Y1 - 2015/10/13

N2 - Numerous human diseases can be associated with fungal infections either as potential causative agents or as a result of changed immune status due to a primary disease. Fungal infections caused by Candida species can vary from mild to severe dependent upon the site of infection, length of exposure, and past medical history. Patients with impaired immune status are at increased risk for chronic fungal infections. Recent epidemiologic studies have revealed the increasing incidence of candidiasis caused by non-albicans species such as Candida parapsilosis. Due to its increasing relevance we chose two distinct C. parapsilosis strains, to describe the cellular innate immune response toward this species. In the first section of our study we compared the interaction of CLIB 214 and GA1 cells with murine and human macrophages. Both strains are commonly used to investigate C. parapsilosis virulence properties. CLIB 214 is a rapidly pseudohyphae-forming strain and GA1 is an isolate that mainly exists in a yeast form. Our results showed, that the phagocyte response was similar in terms of overall uptake, however differences were observed in macrophage migration and engulfment of fungal cells. As C. parapsilosis releases extracellular lipases in order to promote host invasion we further investigated the role of these secreted components during the distinct stages of the phagocytic process. Using a secreted lipase deficient mutant strain and the parental strain GA1 individually and simultaneously, we confirmed that fungal secreted lipases influence the fungi's virulence by detecting altered innate cellular responses. In this study we report that two isolates of a single species can trigger markedly distinct host responses and that lipase secretion plays a role on the cellular level of host-pathogen interactions.

AB - Numerous human diseases can be associated with fungal infections either as potential causative agents or as a result of changed immune status due to a primary disease. Fungal infections caused by Candida species can vary from mild to severe dependent upon the site of infection, length of exposure, and past medical history. Patients with impaired immune status are at increased risk for chronic fungal infections. Recent epidemiologic studies have revealed the increasing incidence of candidiasis caused by non-albicans species such as Candida parapsilosis. Due to its increasing relevance we chose two distinct C. parapsilosis strains, to describe the cellular innate immune response toward this species. In the first section of our study we compared the interaction of CLIB 214 and GA1 cells with murine and human macrophages. Both strains are commonly used to investigate C. parapsilosis virulence properties. CLIB 214 is a rapidly pseudohyphae-forming strain and GA1 is an isolate that mainly exists in a yeast form. Our results showed, that the phagocyte response was similar in terms of overall uptake, however differences were observed in macrophage migration and engulfment of fungal cells. As C. parapsilosis releases extracellular lipases in order to promote host invasion we further investigated the role of these secreted components during the distinct stages of the phagocytic process. Using a secreted lipase deficient mutant strain and the parental strain GA1 individually and simultaneously, we confirmed that fungal secreted lipases influence the fungi's virulence by detecting altered innate cellular responses. In this study we report that two isolates of a single species can trigger markedly distinct host responses and that lipase secretion plays a role on the cellular level of host-pathogen interactions.

KW - Candida parapsilosis

KW - phagocyte response

KW - secreted lipase

KW - co-infection

KW - live cell imaging

U2 - 10.3389/fmicb.2015.01102

DO - 10.3389/fmicb.2015.01102

M3 - Article

C2 - 26528256

SN - 1664-302X

VL - 6

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 1102

ER -

Different Candida parapsilosis clinical isolates and lipase deficient strain trigger an altered cellular immune response

Abstract

Bibliographical note

Keywords

UN SDGs

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