Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro

Francesca Borrelli, Inmaculada Posadas, Raffaele Capasso, Gabriella Aviello, Valeria Ascione, Francesco Capasso

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8 Citations (Scopus)


Caffeic acid phenethyl ester (CAPE), one of the major components of propolis (honeybee resin), has demonstrated a wide spectrum of activities including suppression of eicosanoids by inhibition of cyclooxygenase-1 and cyclooxygenase-2 enzyme activities. The aim of this study was to investigate the effect of CAPE on basal and secretagogues-stimulated gastric acid secretion in vitro. In the isolated, lumen-perfused, stomach preparation of mouse, CAPE (10-100 microM) did not affect the basal gastric acid secretion nor the secretion stimulated by histamine, pentagastrin, isobutyl methylxanthine and high levels of K+. By contrast, CAPE increased the gastric acid secretion induced by the muscarinic receptor agonist, 5-methylfurmethide (5-MEF). CAPE also inhibited the acetylcholinesterase activity in an in vitro colorimetric assay. Eserine (10 microM), a well known acetylcholinesterase inhibitor, also increased 5-MEF-stimulated acid secretion. Our results show that CAPE increases gastric acid secretion stimulated by an acetylcholine agonist receptor likely through inhibition of acetylcholinesterase activity.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
JournalEuropean Journal of Pharmacology
Issue number1-3
Publication statusPublished - 3 Oct 2005

Bibliographical note

This work was supported by Regione Campania and Italian MIUR.


  • 1-Methyl-3-isobutylxanthine
  • Acetylcholinesterase
  • Animals
  • Caffeic Acids
  • Cholinesterase Inhibitors
  • Dose-Response Relationship, Drug
  • Gastric Acid
  • Gastrointestinal Agents
  • Histamine
  • In Vitro Techniques
  • Male
  • Mice
  • Mice, Inbred ICR
  • Muscarine
  • Nifedipine
  • Pentagastrin
  • Phenylethyl Alcohol
  • Physostigmine
  • Potassium
  • Stomach
  • Journal Article
  • Research Support, Non-U.S. Gov't


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