Effect of HBT on the stability of laccase during the decolourization of textile wastewaters

Rim Khlifi, Sami Sayadi, Lassaad Belbahri, Stephen Woodward, Tahar Mechichi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


BACKGROUND: This study concerns the assessment of laccase stability during textile industry effluent treatment by laccase in the presence of a mediator

RESULTS: The effects of 1-hydroxybenzotriazole (HBT) and textile wastewater on the stability of a crude laccase prepared from cultures of Trametes trogii during the process of textile industry effluent decolourization were determined. HBT was essential for decolourization of effluent by laccase. Decolourization efficiency decreased when effluent was repeatedly added to the reaction mixture, suggesting inhibition of laccase during the process. Stability of the laccase was not affected by different concentrations of effluent, but inactivation was proportional to HBT concentration.

CONCLUSION: Experimental results showed that a redox mediator such as HBT was required for high decolourization rate of textile industry effluent; however free radicals generated from this compound inactivated the laccase used in the process. (C) 2009 Society of Chemical Industry

Original languageEnglish
Pages (from-to)1828-1833
Number of pages6
JournalJournal of Chemical Technology & Biotechnology
Issue number12
Early online date14 Aug 2009
Publication statusPublished - Dec 2009


  • laccase
  • stability
  • textile industry
  • dyes
  • mediator
  • 1-hydroxybenzotriazole
  • lignin model compounds
  • N-hydroxy compounds
  • brilliant-blue-R
  • fungal laccases
  • catalyzed oxidation
  • redox mediators
  • trametes-trogii
  • bisphenol-A
  • enzyme


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