Effect of intraperitoneally administered IL-1β-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

Scott Peddie; Pamela E. McLauchlan; Anthony E. Ellis; Christopher J. Secombes

doi:10.3354/dao056195

Effect of intraperitoneally administered IL-1β-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

Scott Peddie, Pamela E. McLauchlan, Anthony E. Ellis, Christopher J. Secombes^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The present work provides the first information concerning the immunostimulatory activity of trout interleukin (IL)-1β-derived peptides in vivo. Previous studies have demonstrated the ability of 2 such peptides, referred to as P1 and P3, to up-regulate a range of important immune parameters in vitro. P1 corresponds to fragment 146-157 (YVTPVPIETEAR) of the trout sequence and is analogous to a biologically active mammalian IL-1β-derived peptide, whilst P3 was synthesised to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. Optimal migration of peritoneal leucocytes, peptide induced phagocytosis and intracellular respiratory burst activity occurred following intraperitoneal injection of 3.0 μmol of P3. Furthermore, resistance to viral haemorrhagic septicaemia virus (VHSV) was soon augmented (2 d) post-injection of P3.

Original language	English
Pages (from-to)	195-200
Number of pages	6
Journal	Diseases of Aquatic Organisms
Volume	56
Issue number	3
DOIs	https://doi.org/10.3354/dao056195
Publication status	Published - 24 Oct 2003

Keywords

Disease resistance
Immunomodulation
Interleukin-1β
Phagocytosis
Respiratory burst
VHS

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title = "Effect of intraperitoneally administered IL-1β-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss",

abstract = "The present work provides the first information concerning the immunostimulatory activity of trout interleukin (IL)-1β-derived peptides in vivo. Previous studies have demonstrated the ability of 2 such peptides, referred to as P1 and P3, to up-regulate a range of important immune parameters in vitro. P1 corresponds to fragment 146-157 (YVTPVPIETEAR) of the trout sequence and is analogous to a biologically active mammalian IL-1β-derived peptide, whilst P3 was synthesised to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. Optimal migration of peritoneal leucocytes, peptide induced phagocytosis and intracellular respiratory burst activity occurred following intraperitoneal injection of 3.0 μmol of P3. Furthermore, resistance to viral haemorrhagic septicaemia virus (VHSV) was soon augmented (2 d) post-injection of P3.",

keywords = "Disease resistance, Immunomodulation, Interleukin-1β, Phagocytosis, Respiratory burst, VHS",

author = "Scott Peddie and McLauchlan, {Pamela E.} and Ellis, {Anthony E.} and Secombes, {Christopher J.}",

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AU - Peddie, Scott

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AU - Ellis, Anthony E.

AU - Secombes, Christopher J.

PY - 2003/10/24

Y1 - 2003/10/24

N2 - The present work provides the first information concerning the immunostimulatory activity of trout interleukin (IL)-1β-derived peptides in vivo. Previous studies have demonstrated the ability of 2 such peptides, referred to as P1 and P3, to up-regulate a range of important immune parameters in vitro. P1 corresponds to fragment 146-157 (YVTPVPIETEAR) of the trout sequence and is analogous to a biologically active mammalian IL-1β-derived peptide, whilst P3 was synthesised to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. Optimal migration of peritoneal leucocytes, peptide induced phagocytosis and intracellular respiratory burst activity occurred following intraperitoneal injection of 3.0 μmol of P3. Furthermore, resistance to viral haemorrhagic septicaemia virus (VHSV) was soon augmented (2 d) post-injection of P3.

AB - The present work provides the first information concerning the immunostimulatory activity of trout interleukin (IL)-1β-derived peptides in vivo. Previous studies have demonstrated the ability of 2 such peptides, referred to as P1 and P3, to up-regulate a range of important immune parameters in vitro. P1 corresponds to fragment 146-157 (YVTPVPIETEAR) of the trout sequence and is analogous to a biologically active mammalian IL-1β-derived peptide, whilst P3 was synthesised to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. Optimal migration of peritoneal leucocytes, peptide induced phagocytosis and intracellular respiratory burst activity occurred following intraperitoneal injection of 3.0 μmol of P3. Furthermore, resistance to viral haemorrhagic septicaemia virus (VHSV) was soon augmented (2 d) post-injection of P3.

KW - Disease resistance

KW - Immunomodulation

KW - Interleukin-1β

KW - Phagocytosis

KW - Respiratory burst

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ER -

Effect of intraperitoneally administered IL-1β-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

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Keywords

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