Abstract
PURPOSE. This study evaluated the effect of transforming growth factor (TGF)-beta 2 and anti-TGF-beta 2 antibody in a rodent model of posterior capsule opacification (PCO).
METHODS. An extracapsular lens extraction (ECLE) was performed in 72 Sprague - Dawley rats. At the end of the procedure, 10 mu L TGF-beta 2 (TGF-beta 2-treated group), fetal calf serum (FCS)/phosphate-buffered saline (PBS; FCS/PBS-treated control group), a human monoclonal TGF-beta 2 antibody ( anti - TGF-beta 2 treated group), or a null control IgG4 antibody ( null antibody treated control group) was injected into the capsule. Animals were killed 3 and 14 days postoperatively. Eyes were evaluated clinically prior to euthanatization, then enucleated and processed for light microscopy and immunohistochemistry afterward. PCO was evaluated clinically and histopathologically. Student's t-test and chi(2) were used to assess differences between groups.
RESULTS. There were no statistically significant clinical or histopathological differences in degree of PCO between the TGF-beta 2 - and FCS/PBS-treated groups at 3 and 14 days after ECLE. Nor were there differences between the anti - TGF-beta 2 - and the null antibody - treated groups, with the exception of the histopathology score for capsule wrinkling 3 days after ECLE ( P = 0.02). alpha-Smooth-muscle actin staining was observed in the lens capsular bag only in areas where there was close contact with the iris.
CONCLUSIONS. No sustained effect of TGF-beta 2 or anti - TGF-beta 2 antibody on PCO was found in rodents at the dose and timing administered in this study. Iris cells may play a role in the process of epithelial mesenchymal transition linked to PCO.
Original language | English |
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Pages (from-to) | 4260-4266 |
Number of pages | 6 |
Journal | Investigative Ophthalmology & Visual Science |
Volume | 46 |
DOIs | |
Publication status | Published - 2005 |
Keywords
- GROWTH-FACTOR-BETA
- LENS EPITHELIAL-CELLS
- SMOOTH MUSCLE ACTIN
- HUMAN AQUEOUS-HUMOR
- TGF-BETA
- EXTRACELLULAR-MATRIX
- CATARACT
- PROLIFERATION
- EXPRESSION
- TRANSDIFFERENTIATION
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Microscopy and Histology
Debbie Wilkinson (Manager) & Gillian Milne (Manager)
Medical SciencesResearch Facilities: Facility