Effects of dietary macronutrients and body composition on glucose homeostasis in mice

Sumei Hu, Jacques Togo, Lu Wang, Yingga Wu, Dengbao Yang, Yanchao Xu, Li Li, Baoguo Li, Min Li, Jianbo Li, Guanlin Wang, Xueying Zhang, Chaoqun Niu, Mohsen Mazidi, Alex Douglas, John R. Speakman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
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As a major health issue, obesity is linked with elevated risk of type 2 diabetes. However, whether disrupted glucose homeostasis is due to altered body composition alone, or whether dietary macronutrients play an additional role, independent of their impact on body composition, remains unclear. We investigated the associations between macronutrients, body composition, blood hormones and glucose homeostasis. We fed C57BL/6N mice 29 different diets with variable macronutrients for 12 weeks. After 10 weeks, intraperitoneal glucose tolerance tests were performed. Generalized linear models were generated to evaluate the impacts of macronutrients, body composition and blood hormones on glucose homeostasis. The area under the glucose curve (AUC) was strongly associated with body fat mass, but not dietary macronutrients. AUC was significantly associated with fasting insulin levels. Six genes from transcriptomic analysis of epididymal white adipose tissue and subcutaneous white adipose tissue were significantly associated with AUC. These genes may encode secreted proteins that play important previously unanticipated roles in glucose homeostasis.

Original languageEnglish
Article numbernwaa177
Number of pages11
JournalNational Science Review
Issue number1
Early online date6 Aug 2020
Publication statusPublished - 1 Jan 2021

Bibliographical note

This work was supported by the Chinese Academy of Sciences Strategic Programs (XDA12030209 and XDB13030100), the 1000 Talents Program and a Wolfson Merit Award to J.R.S


  • body fat mass
  • dietary macronutrients
  • fasting blood glucose
  • glucose tolerance
  • mice


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