Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts: Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism

J E HESKETH, Gillian Patricia Campbell

Research output: Contribution to journalArticlepeer-review

28 Citations (Web of Science)

Abstract

The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels.
Original languageEnglish
Pages (from-to)533-541
Number of pages9
JournalBioscience Reports
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 1987

Keywords

  • insulin
  • protein synthesis
  • G-protein
  • diacylglycerol
  • phospholipase C
  • signal transduction

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