Effects of temperature on Paramoeba perurans growth in culture and the associated microbial community

O. Benedicenti* (Corresponding Author), C. J. Secombes, C. Collins*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
9 Downloads (Pure)


Population growth, in vitro, of three Paramoeba perurans cultures, one polyclonal (G) and two clonal (B8, CE6, derived from G), previously shown to differ in virulence (B8 > G > CE6), was compared at 10 and 15 °C. B8 showed a significantly higher increase in attached and in suspended amoebae over time at 15 and 10 °C, respectively. CE6 and G also had significantly higher numbers of suspended amoebae at 10 °C compared with 15 °C at experiment termination. However, in contrast to B8, numbers of attached amoebae were significantly higher at 10 °C in CE6 but showed a similar trend in G at the end of the experiment. Numbers of both suspended and attached amoebae were lower in B8 compared with CE6 and G. Significant differences in bacterial community composition and/or relative abundances were found, between cultures, between temperatures and between the same culture with and without amoebae, based on 16S rRNA Illumina MiSeq sequencing. Bacterial diversity was lower in B8 and CE6 compared with G, possibly reflecting selection during clonal isolation. The results indicate that polyclonal P. perurans populations may contain amoebae displaying different growth dynamics. Further studies are required to determine if these differences are linked to differences seen in the bacterial communities.

Original languageEnglish
Pages (from-to)533-542
Number of pages10
Issue number4
Early online date25 Oct 2018
Publication statusPublished - Apr 2019

Bibliographical note

The authors thank Dr Una McCarthy for reading of the manuscript and for invaluable advice regarding the amoebae in vitro experiment, and Dr Graeme Nicol for advice on the microbial communities experiment. The MiSeq data were generated by RTL Genomics, Lubbock, TX, USA.


  • 16S rRNA
  • Amoebic gill disease
  • Paramoeba perurans
  • temperature
  • SALAR L.
  • AGD


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