Electrical estimulation of retinal pigment epithelial cells

Olga Lucia Gamboa; Jin Pu; John Townend; John V. Forrester; Min Zhao; Colin McCaig; Noemi Lois

doi:10.1016/j.exer.2010.04.018

Electrical estimulation of retinal pigment epithelial cells

Olga Lucia Gamboa, Jin Pu, John Townend, John V. Forrester, Min Zhao, Colin McCaig, Noemi Lois^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

We investigated and characterized the effect of externally applied electric fields (EF) on retinal pigment epithelial (RPE) cells by exposing primary cultures of human RPE cells (hRPE) and those from the ARPE19 immortalized cell line to various strengths of EF (EF-treated cells) or to no EF (control cells) under different conditions including presence or absence of serum and gelatin and following wounding. We evaluated changes in RPE cell behavior in response to EF by using a computer based image capture and analysis system (Metamorph). We found that RPE cells responded to externally applied EFs by preferential orientation perpendicular to the EF vector, directed migration towards the anode, and faster translocation rate than control, untreated cells. These responses were voltage-dependent. Responses were observed even at low voltages, of 50-300 mV. Furthermore, the migration of hRPE cell sheets generated by wounding of confluent monolayers of cells at early and late confluence could be manipulated by the application of EF, with directed migration towards the anode observed at both sides of the wounded hRPE. In conclusion, RPE cell behaviour can be controlled by an externally applied EF. The potential for externally applied EF to be used as a therapeutic strategy in the management of selected retinal diseases warrants further investigation.

Original language	English
Pages (from-to)	195-204
Number of pages	10
Journal	Experimental Eye Research
Volume	91
Issue number	2
Early online date	10 May 2010
DOIs	https://doi.org/10.1016/j.exer.2010.04.018
Publication status	Published - Aug 2010

Bibliographical note

Acknowledgements The authors would like to thank Dr. Isabel J. Crane for providing hRPE cells and Carol Wallace and Rosie Fordyce for their technical help. This work was supported by the New and Emerging Applications of Technology (NEAT) program, National Institute for Health Research, UK, and The Royal College of Surgeons of Edinburgh

Keywords

electric currents
electric fields
retina
retinal diseases
retinal pigment epithelium

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Electrical estimulation of retinal pigment epithelial cells",

abstract = "We investigated and characterized the effect of externally applied electric fields (EF) on retinal pigment epithelial (RPE) cells by exposing primary cultures of human RPE cells (hRPE) and those from the ARPE19 immortalized cell line to various strengths of EF (EF-treated cells) or to no EF (control cells) under different conditions including presence or absence of serum and gelatin and following wounding. We evaluated changes in RPE cell behavior in response to EF by using a computer based image capture and analysis system (Metamorph). We found that RPE cells responded to externally applied EFs by preferential orientation perpendicular to the EF vector, directed migration towards the anode, and faster translocation rate than control, untreated cells. These responses were voltage-dependent. Responses were observed even at low voltages, of 50-300 mV. Furthermore, the migration of hRPE cell sheets generated by wounding of confluent monolayers of cells at early and late confluence could be manipulated by the application of EF, with directed migration towards the anode observed at both sides of the wounded hRPE. In conclusion, RPE cell behaviour can be controlled by an externally applied EF. The potential for externally applied EF to be used as a therapeutic strategy in the management of selected retinal diseases warrants further investigation.",

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note = "Acknowledgements The authors would like to thank Dr. Isabel J. Crane for providing hRPE cells and Carol Wallace and Rosie Fordyce for their technical help. This work was supported by the New and Emerging Applications of Technology (NEAT) program, National Institute for Health Research, UK, and The Royal College of Surgeons of Edinburgh",

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AU - Gamboa, Olga Lucia

AU - Pu, Jin

AU - Townend, John

AU - Forrester, John V.

AU - Zhao, Min

AU - McCaig, Colin

AU - Lois, Noemi

N1 - Acknowledgements The authors would like to thank Dr. Isabel J. Crane for providing hRPE cells and Carol Wallace and Rosie Fordyce for their technical help. This work was supported by the New and Emerging Applications of Technology (NEAT) program, National Institute for Health Research, UK, and The Royal College of Surgeons of Edinburgh

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N2 - We investigated and characterized the effect of externally applied electric fields (EF) on retinal pigment epithelial (RPE) cells by exposing primary cultures of human RPE cells (hRPE) and those from the ARPE19 immortalized cell line to various strengths of EF (EF-treated cells) or to no EF (control cells) under different conditions including presence or absence of serum and gelatin and following wounding. We evaluated changes in RPE cell behavior in response to EF by using a computer based image capture and analysis system (Metamorph). We found that RPE cells responded to externally applied EFs by preferential orientation perpendicular to the EF vector, directed migration towards the anode, and faster translocation rate than control, untreated cells. These responses were voltage-dependent. Responses were observed even at low voltages, of 50-300 mV. Furthermore, the migration of hRPE cell sheets generated by wounding of confluent monolayers of cells at early and late confluence could be manipulated by the application of EF, with directed migration towards the anode observed at both sides of the wounded hRPE. In conclusion, RPE cell behaviour can be controlled by an externally applied EF. The potential for externally applied EF to be used as a therapeutic strategy in the management of selected retinal diseases warrants further investigation.

AB - We investigated and characterized the effect of externally applied electric fields (EF) on retinal pigment epithelial (RPE) cells by exposing primary cultures of human RPE cells (hRPE) and those from the ARPE19 immortalized cell line to various strengths of EF (EF-treated cells) or to no EF (control cells) under different conditions including presence or absence of serum and gelatin and following wounding. We evaluated changes in RPE cell behavior in response to EF by using a computer based image capture and analysis system (Metamorph). We found that RPE cells responded to externally applied EFs by preferential orientation perpendicular to the EF vector, directed migration towards the anode, and faster translocation rate than control, untreated cells. These responses were voltage-dependent. Responses were observed even at low voltages, of 50-300 mV. Furthermore, the migration of hRPE cell sheets generated by wounding of confluent monolayers of cells at early and late confluence could be manipulated by the application of EF, with directed migration towards the anode observed at both sides of the wounded hRPE. In conclusion, RPE cell behaviour can be controlled by an externally applied EF. The potential for externally applied EF to be used as a therapeutic strategy in the management of selected retinal diseases warrants further investigation.

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Electrical estimulation of retinal pigment epithelial cells

Abstract

Bibliographical note

Keywords

UN SDGs

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