Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice

Simon Clare, Victoria John, Alan W Walker, Jennifer L Hill, Cei Abreu-Goodger, Christine Hale, David Goulding, Trevor D Lawley, Pietro Mastroeni, Gadi Frankel, Anton J Enright, Elena Vigorito, Gordon Dougan

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


MicroRNAs (miRNAs) are small noncoding molecules that control gene expression posttranscriptionally, with microRNA-155 (miR-155) one of the first to be implicated in immune regulation. Here, we show that miR-155-deficient mice are less able to eradicate a mucosal Citrobacter rodentium infection than wild-type C57BL/6 mice. miR-155-deficient mice exhibited prolonged colonization associated with a higher C. rodentium burden in gastrointestinal tissue and spread into systemic tissues. Germinal center formation and humoral immune responses against C. rodentium were severely impaired in infected miR-155-deficient mice. A similarly susceptible phenotype was observed in μMT mice reconstituted with miR-155-deficient B cells, indicating that miR-155 is required intrinsically for mediating protection against this predominantly luminal bacterial pathogen.
Original languageEnglish
Pages (from-to)723-732
Number of pages10
JournalInfection and Immunity
Issue number3
Early online date21 Dec 2012
Publication statusPublished - Mar 2013


  • Animals
  • Citrobacter rodentium
  • Colitis
  • Enterobacteriaceae Infections
  • Female
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • MicroRNAs
  • RNA, Ribosomal, 16S
  • Real-Time Polymerase Chain Reaction
  • Transcriptome


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