Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A

Yating Liu, Li Su, Qing Fang, Jioji Tabudravu, Xiaohui Yang, Kirstie Rickaby, Laurent Trembleau, Kwaku Kyeremeh, Zixin Deng, Hai Deng* (Corresponding Author), Yi Yu (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)
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Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis.
Original languageEnglish
Pages (from-to)16323-16328
Number of pages6
JournalJournal of Organic Chemistry
Issue number24
Early online date15 Nov 2019
Publication statusPublished - 20 Dec 2019

Bibliographical note

We thank the National Key Research and Development Program of China (2018YFA0900400), the National Natural Science Foundation of China (31570033, 31811530299, and 31870035 to Y.Y.), the Royal Society-NSFC Newton Mobility Grant Award (IEC\NSFC\170617 to H.D. and Y.Y.), Biotechnology and Biological Sciences Research Council UK (H.D., K.R., and L.T., BB/R00479X/1), the Elphinstone
Scholarship of University of Aberdeen (Q.F.), Leverhulme Trust-Royal Society Africa award (AA090088), and Medical Research Council UK Africa Future leader (K.K. and H.D., MR/S00520X/1) for financial support.


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