Evolution of pathogenicity and sexual reproduction in eight Candida genomes

Geraldine Butler; Matthew D Rasmussen; Michael F Lin; Manuel A S Santos; Sharadha Sakthikumar; Carol A Munro; Esther Rheinbay; Manfred Grabherr; Anja Forche; Jennifer L Reedy; Ino Agrafioti; Martha B Arnaud; Steven Bates; Alistair J P Brown; Sascha Brunke; Maria C Costanzo; David A Fitzpatrick; Piet W J de Groot; David Harris; Lois L Hoyer; Bernhard Hube; Frans M Klis; Chinnappa Kodira; Nicola Lennard; Mary E Logue; Ronny Martin; Aaron M Neiman; Elissavet Nikolaou; Michael A Quail; Janet Quinn; Maria C Santos; Florian F Schmitzberger; Gavin Sherlock; Prachi Shah; Kevin A T Silverstein; Marek S Skrzypek; David Soll; Rodney Staggs; Ian Stansfield; Michael P H Stumpf; Peter E Sudbery; Thyagarajan Srikantha; Qiandong Zeng; Judith Berman; Matthew Berriman; Joseph Heitman; Neil A R Gow; Michael C Lorenz; Bruce W Birren; Manolis Kellis; Christina A Cuomo

doi:10.1038/nature08064

Evolution of pathogenicity and sexual reproduction in eight Candida genomes

Geraldine Butler, Matthew D Rasmussen, Michael F Lin, Manuel A S Santos, Sharadha Sakthikumar, Carol A Munro, Esther Rheinbay, Manfred Grabherr, Anja Forche, Jennifer L Reedy, Ino Agrafioti, Martha B Arnaud, Steven Bates, Alistair J P Brown, Sascha Brunke, Maria C Costanzo, David A Fitzpatrick, Piet W J de Groot, David Harris, Lois L HoyerBernhard Hube, Frans M Klis, Chinnappa Kodira, Nicola Lennard, Mary E Logue, Ronny Martin, Aaron M Neiman, Elissavet Nikolaou, Michael A Quail, Janet Quinn, Maria C Santos, Florian F Schmitzberger, Gavin Sherlock, Prachi Shah, Kevin A T Silverstein, Marek S Skrzypek, David Soll, Rodney Staggs, Ian Stansfield, Michael P H Stumpf, Peter E Sudbery, Thyagarajan Srikantha, Qiandong Zeng, Judith Berman, Matthew Berriman, Joseph Heitman, Neil A R Gow, Michael C Lorenz, Bruce W Birren, Manolis Kellis, Christina A Cuomo

Medical Sciences

Research output: Contribution to journal › Article › peer-review

826 Citations (Scopus)

Abstract

Candida species are the most common cause of opportunistic fungal infection worldwide. Here we report the genome sequences of six Candida species and compare these and related pathogens and non-pathogens. There are significant expansions of cell wall, secreted and transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts are homozygous in three diploid species, possibly resulting from recent recombination events. Surprisingly, key components of the mating and meiosis pathways are missing from several species. These include major differences at the mating-type loci (MTL); Lodderomyces elongisporus lacks MTL, and components of the a1/2 cell identity determinant were lost in other species, raising questions about how mating and cell types are controlled. Analysis of the CUG leucine-to-serine genetic-code change reveals that 99% of ancestral CUG codons were erased and new ones arose elsewhere. Lastly, we revise the Candida albicans gene catalogue, identifying many new genes.

Original language	English
Pages (from-to)	657-662
Number of pages	6
Journal	Nature
Volume	459
Issue number	7247
Early online date	24 May 2009
DOIs	https://doi.org/10.1038/nature08064
Publication status	Published - 4 Jun 2009

Bibliographical note

Open Access via PMC2834264

Keywords

Candida
codon
conserved sequence
diploidy
evolution, molecular
genes, fungal
genome, fungal
meiosis
polymorphism, genetic
reproduction
saccharomyces
virulence

Access to Document

10.1038/nature08064

Cite this

Butler, G., Rasmussen, M. D., Lin, M. F., Santos, M. A. S., Sakthikumar, S., Munro, C. A., Rheinbay, E., Grabherr, M., Forche, A., Reedy, J. L., Agrafioti, I., Arnaud, M. B., Bates, S., Brown, A. J. P., Brunke, S., Costanzo, M. C., Fitzpatrick, D. A., de Groot, P. W. J., Harris, D., ... Cuomo, C. A. (2009). Evolution of pathogenicity and sexual reproduction in eight Candida genomes. Nature, 459(7247), 657-662. https://doi.org/10.1038/nature08064

Butler, G, Rasmussen, MD, Lin, MF, Santos, MAS, Sakthikumar, S, Munro, CA, Rheinbay, E, Grabherr, M, Forche, A, Reedy, JL, Agrafioti, I, Arnaud, MB, Bates, S, Brown, AJP, Brunke, S, Costanzo, MC, Fitzpatrick, DA, de Groot, PWJ, Harris, D, Hoyer, LL, Hube, B, Klis, FM, Kodira, C, Lennard, N, Logue, ME, Martin, R, Neiman, AM, Nikolaou, E, Quail, MA, Quinn, J, Santos, MC, Schmitzberger, FF, Sherlock, G, Shah, P, Silverstein, KAT, Skrzypek, MS, Soll, D, Staggs, R, Stansfield, I, Stumpf, MPH, Sudbery, PE, Srikantha, T, Zeng, Q, Berman, J, Berriman, M, Heitman, J, Gow, NAR, Lorenz, MC, Birren, BW, Kellis, M & Cuomo, CA 2009, 'Evolution of pathogenicity and sexual reproduction in eight Candida genomes', Nature, vol. 459, no. 7247, pp. 657-662. https://doi.org/10.1038/nature08064

@article{2856c22d4c97440d9b9d2c773d5aff92,

title = "Evolution of pathogenicity and sexual reproduction in eight Candida genomes",

abstract = "Candida species are the most common cause of opportunistic fungal infection worldwide. Here we report the genome sequences of six Candida species and compare these and related pathogens and non-pathogens. There are significant expansions of cell wall, secreted and transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts are homozygous in three diploid species, possibly resulting from recent recombination events. Surprisingly, key components of the mating and meiosis pathways are missing from several species. These include major differences at the mating-type loci (MTL); Lodderomyces elongisporus lacks MTL, and components of the a1/2 cell identity determinant were lost in other species, raising questions about how mating and cell types are controlled. Analysis of the CUG leucine-to-serine genetic-code change reveals that 99% of ancestral CUG codons were erased and new ones arose elsewhere. Lastly, we revise the Candida albicans gene catalogue, identifying many new genes.",

keywords = "Candida, codon, conserved sequence, diploidy, evolution, molecular, genes, fungal, genome, fungal, meiosis, polymorphism, genetic, reproduction, saccharomyces, virulence",

author = "Geraldine Butler and Rasmussen, {Matthew D} and Lin, {Michael F} and Santos, {Manuel A S} and Sharadha Sakthikumar and Munro, {Carol A} and Esther Rheinbay and Manfred Grabherr and Anja Forche and Reedy, {Jennifer L} and Ino Agrafioti and Arnaud, {Martha B} and Steven Bates and Brown, {Alistair J P} and Sascha Brunke and Costanzo, {Maria C} and Fitzpatrick, {David A} and {de Groot}, {Piet W J} and David Harris and Hoyer, {Lois L} and Bernhard Hube and Klis, {Frans M} and Chinnappa Kodira and Nicola Lennard and Logue, {Mary E} and Ronny Martin and Neiman, {Aaron M} and Elissavet Nikolaou and Quail, {Michael A} and Janet Quinn and Santos, {Maria C} and Schmitzberger, {Florian F} and Gavin Sherlock and Prachi Shah and Silverstein, {Kevin A T} and Skrzypek, {Marek S} and David Soll and Rodney Staggs and Ian Stansfield and Stumpf, {Michael P H} and Sudbery, {Peter E} and Thyagarajan Srikantha and Qiandong Zeng and Judith Berman and Matthew Berriman and Joseph Heitman and Gow, {Neil A R} and Lorenz, {Michael C} and Birren, {Bruce W} and Manolis Kellis and Cuomo, {Christina A}",

note = "Open Access via PMC2834264",

year = "2009",

month = jun,

day = "4",

doi = "10.1038/nature08064",

language = "English",

volume = "459",

pages = "657--662",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "7247",

}

TY - JOUR

T1 - Evolution of pathogenicity and sexual reproduction in eight Candida genomes

AU - Butler, Geraldine

AU - Rasmussen, Matthew D

AU - Lin, Michael F

AU - Santos, Manuel A S

AU - Sakthikumar, Sharadha

AU - Munro, Carol A

AU - Rheinbay, Esther

AU - Grabherr, Manfred

AU - Forche, Anja

AU - Reedy, Jennifer L

AU - Agrafioti, Ino

AU - Arnaud, Martha B

AU - Bates, Steven

AU - Brown, Alistair J P

AU - Brunke, Sascha

AU - Costanzo, Maria C

AU - Fitzpatrick, David A

AU - de Groot, Piet W J

AU - Harris, David

AU - Hoyer, Lois L

AU - Hube, Bernhard

AU - Klis, Frans M

AU - Kodira, Chinnappa

AU - Lennard, Nicola

AU - Logue, Mary E

AU - Martin, Ronny

AU - Neiman, Aaron M

AU - Nikolaou, Elissavet

AU - Quail, Michael A

AU - Quinn, Janet

AU - Santos, Maria C

AU - Schmitzberger, Florian F

AU - Sherlock, Gavin

AU - Shah, Prachi

AU - Silverstein, Kevin A T

AU - Skrzypek, Marek S

AU - Soll, David

AU - Staggs, Rodney

AU - Stansfield, Ian

AU - Stumpf, Michael P H

AU - Sudbery, Peter E

AU - Srikantha, Thyagarajan

AU - Zeng, Qiandong

AU - Berman, Judith

AU - Berriman, Matthew

AU - Heitman, Joseph

AU - Gow, Neil A R

AU - Lorenz, Michael C

AU - Birren, Bruce W

AU - Kellis, Manolis

AU - Cuomo, Christina A

N1 - Open Access via PMC2834264

PY - 2009/6/4

Y1 - 2009/6/4

N2 - Candida species are the most common cause of opportunistic fungal infection worldwide. Here we report the genome sequences of six Candida species and compare these and related pathogens and non-pathogens. There are significant expansions of cell wall, secreted and transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts are homozygous in three diploid species, possibly resulting from recent recombination events. Surprisingly, key components of the mating and meiosis pathways are missing from several species. These include major differences at the mating-type loci (MTL); Lodderomyces elongisporus lacks MTL, and components of the a1/2 cell identity determinant were lost in other species, raising questions about how mating and cell types are controlled. Analysis of the CUG leucine-to-serine genetic-code change reveals that 99% of ancestral CUG codons were erased and new ones arose elsewhere. Lastly, we revise the Candida albicans gene catalogue, identifying many new genes.

AB - Candida species are the most common cause of opportunistic fungal infection worldwide. Here we report the genome sequences of six Candida species and compare these and related pathogens and non-pathogens. There are significant expansions of cell wall, secreted and transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts are homozygous in three diploid species, possibly resulting from recent recombination events. Surprisingly, key components of the mating and meiosis pathways are missing from several species. These include major differences at the mating-type loci (MTL); Lodderomyces elongisporus lacks MTL, and components of the a1/2 cell identity determinant were lost in other species, raising questions about how mating and cell types are controlled. Analysis of the CUG leucine-to-serine genetic-code change reveals that 99% of ancestral CUG codons were erased and new ones arose elsewhere. Lastly, we revise the Candida albicans gene catalogue, identifying many new genes.

KW - Candida

KW - codon

KW - conserved sequence

KW - diploidy

KW - evolution, molecular

KW - genes, fungal

KW - genome, fungal

KW - meiosis

KW - polymorphism, genetic

KW - reproduction

KW - saccharomyces

KW - virulence

U2 - 10.1038/nature08064

DO - 10.1038/nature08064

M3 - Article

C2 - 19465905

SN - 0028-0836

VL - 459

SP - 657

EP - 662

JO - Nature

JF - Nature

IS - 7247

ER -

Evolution of pathogenicity and sexual reproduction in eight Candida genomes

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this