Exploring a Streptomyces wax synthase using acyl-SNACs as donor substrates

Federica Casolari; Saad Alrashdi; Reuben Carr; Hai Deng

doi:10.1039/d3cb00107e

Exploring a Streptomyces wax synthase using acyl-SNACs as donor substrates

Federica Casolari, Saad Alrashdi, Reuben Carr, Hai Deng^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The demand of fragrance and food industries for short/branched wax esters is increasing due to their rich scent and low toxicity. Wax synthase and acyl-CoA:diacylglycerol O-acyltransferase (WS/DGAT) are a family of bacterial enzymes capable of catalysing the production of wax esters. Here, we report that a WS/DGAT from Streptomyces coelicolor is able to mediate the reactions between alcohol acceptors and synthetic acyl-donor mimics, acyl-SNACs. The enzyme displayed considerable substrate tolerance towards acyl-donors with structural diversity. Structural modelling-guided site directed mutagenesis resulted in a variant, L25F, the catalytic efficiency of which was improved toward aromatic, short-linear, and branched acyl-donors compared to the wild type.

Original language	English
Pages (from-to)	742-747
Number of pages	6
Journal	RSC Chemical Biology
Volume	4
Issue number	10
Early online date	14 Aug 2023
DOIs	https://doi.org/10.1039/d3cb00107e
Publication status	Published - 1 Oct 2023

Bibliographical note

Funding Information:
The research was funded by the IBioIC PhD studentship (F. C.), the College of Science and Arts, Jouf University, King Khaled Road, Kingdom of Saudi Arabia, and the Royal Embassy of Saudi Arabia Cultural Bureau in the UK for the PhD scholarship (S. A.). H. D. is grateful to the Royal Society – NSFC International Exchanges Award (IEC\NSFC\211349), Biotechnology and Biological Sciences Research Council UK (BB/R00479X/1), the Royal Society of Edinburgh-NSFC Joint Project (947_Deng) and the National Natural Science Foundation of China (31929001).

Data Availability Statement

Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3cb00107e

Access to Document

10.1039/d3cb00107eLicence: CC BY-NC

Casolari_etal_RSCCB_Exploring_A_Streptomyces_VoR
This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. https://creativecommons.org/licenses/by-nc/3.0/
Final published version, 0.98 MBLicence: CC BY-NC

Cite this

@article{8a801a3c5d95435bab6b362773a5e53d,

title = "Exploring a Streptomyces wax synthase using acyl-SNACs as donor substrates",

abstract = "The demand of fragrance and food industries for short/branched wax esters is increasing due to their rich scent and low toxicity. Wax synthase and acyl-CoA:diacylglycerol O-acyltransferase (WS/DGAT) are a family of bacterial enzymes capable of catalysing the production of wax esters. Here, we report that a WS/DGAT from Streptomyces coelicolor is able to mediate the reactions between alcohol acceptors and synthetic acyl-donor mimics, acyl-SNACs. The enzyme displayed considerable substrate tolerance towards acyl-donors with structural diversity. Structural modelling-guided site directed mutagenesis resulted in a variant, L25F, the catalytic efficiency of which was improved toward aromatic, short-linear, and branched acyl-donors compared to the wild type.",

author = "Federica Casolari and Saad Alrashdi and Reuben Carr and Hai Deng",

note = "Funding Information: The research was funded by the IBioIC PhD studentship (F. C.), the College of Science and Arts, Jouf University, King Khaled Road, Kingdom of Saudi Arabia, and the Royal Embassy of Saudi Arabia Cultural Bureau in the UK for the PhD scholarship (S. A.). H. D. is grateful to the Royal Society – NSFC International Exchanges Award (IEC\NSFC\211349), Biotechnology and Biological Sciences Research Council UK (BB/R00479X/1), the Royal Society of Edinburgh-NSFC Joint Project (947_Deng) and the National Natural Science Foundation of China (31929001). ",

year = "2023",

month = oct,

day = "1",

doi = "10.1039/d3cb00107e",

language = "English",

volume = "4",

pages = "742--747",

journal = "RSC Chemical Biology",

issn = "2633-0679",

publisher = "Royal Society of Chemistry",

number = "10",

}

TY - JOUR

T1 - Exploring a Streptomyces wax synthase using acyl-SNACs as donor substrates

AU - Casolari, Federica

AU - Alrashdi, Saad

AU - Carr, Reuben

AU - Deng, Hai

N1 - Funding Information: The research was funded by the IBioIC PhD studentship (F. C.), the College of Science and Arts, Jouf University, King Khaled Road, Kingdom of Saudi Arabia, and the Royal Embassy of Saudi Arabia Cultural Bureau in the UK for the PhD scholarship (S. A.). H. D. is grateful to the Royal Society – NSFC International Exchanges Award (IEC\NSFC\211349), Biotechnology and Biological Sciences Research Council UK (BB/R00479X/1), the Royal Society of Edinburgh-NSFC Joint Project (947_Deng) and the National Natural Science Foundation of China (31929001).

PY - 2023/10/1

Y1 - 2023/10/1

N2 - The demand of fragrance and food industries for short/branched wax esters is increasing due to their rich scent and low toxicity. Wax synthase and acyl-CoA:diacylglycerol O-acyltransferase (WS/DGAT) are a family of bacterial enzymes capable of catalysing the production of wax esters. Here, we report that a WS/DGAT from Streptomyces coelicolor is able to mediate the reactions between alcohol acceptors and synthetic acyl-donor mimics, acyl-SNACs. The enzyme displayed considerable substrate tolerance towards acyl-donors with structural diversity. Structural modelling-guided site directed mutagenesis resulted in a variant, L25F, the catalytic efficiency of which was improved toward aromatic, short-linear, and branched acyl-donors compared to the wild type.

AB - The demand of fragrance and food industries for short/branched wax esters is increasing due to their rich scent and low toxicity. Wax synthase and acyl-CoA:diacylglycerol O-acyltransferase (WS/DGAT) are a family of bacterial enzymes capable of catalysing the production of wax esters. Here, we report that a WS/DGAT from Streptomyces coelicolor is able to mediate the reactions between alcohol acceptors and synthetic acyl-donor mimics, acyl-SNACs. The enzyme displayed considerable substrate tolerance towards acyl-donors with structural diversity. Structural modelling-guided site directed mutagenesis resulted in a variant, L25F, the catalytic efficiency of which was improved toward aromatic, short-linear, and branched acyl-donors compared to the wild type.

UR - http://www.scopus.com/inward/record.url?scp=85169511736&partnerID=8YFLogxK

U2 - 10.1039/d3cb00107e

DO - 10.1039/d3cb00107e

M3 - Article

AN - SCOPUS:85169511736

SN - 2633-0679

VL - 4

SP - 742

EP - 747

JO - RSC Chemical Biology

JF - RSC Chemical Biology

IS - 10

ER -

Exploring a Streptomyces wax synthase using acyl-SNACs as donor substrates

Abstract

Bibliographical note

Data Availability Statement

Access to Document

Other files and links

Fingerprint

Cite this