Abstract
Fission yeast cut5 mutants cause cytokinesis in the absence of normal nuclear division. We show here that cut5+ is required for both the onset of S phase and the restraint of M phase before the completion of S phase. The primary defects in cut5 mutants occur prior to S phase, but cells suffer lethal damage during M phase. Mitosis and cytokinesis occur in the presence of hydroxyurea or in the double mutant cdc10-cut5 (the cdc10 mutation alone blocks progression from G1 to S). Gene cloning shows that cut5+ is identical to the fission yeast rad4+ gene, which is similar to human XRCC1. The rad4+/cut5+ gene is unique in its positive role for replication/repair and in its negative role for mitosis/cytokinesis. We propose a single/twin chromatid marking model for rad4+/cut5+ function in cell cycle control.
Original language | English |
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Pages (from-to) | 383-393 |
Number of pages | 11 |
Journal | Cell |
Volume | 74 |
Issue number | 2 |
DOIs | |
Publication status | Published - 30 Jul 1993 |
Keywords
- Amino Acid Sequence
- Base Sequence
- Cell Cycle
- Cell Cycle Proteins
- Cell Division
- Cell Nucleus
- Cloning, Molecular
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- Genes, Fungal
- Mitosis
- Models, Genetic
- Molecular Sequence Data
- Nitrogen
- Phenotype
- S Phase
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins
- Transcription Factors
- Transformation, Genetic
- Transglutaminases
- Ultraviolet Rays
- X-Rays