Abstract
Pulmonary arterial hypertension (PAH) is characterized by elevated pulmonary artery pressures and right heart failure. PAH mouse models are instrumental in understanding disease pathophysiology. However, few methods are available to evaluate right cardiac function in small animals. In this study, magnetic resonance imaging (MRI) was used to measure in vivo cardiac dimensions in the Sugen 5416/hypoxia mice model of pulmonary hypertension (PH). PH was induced in C57BL/6 mice by three weeks of exposure to 10% oxygen and VEGF receptor inhibition (20 mg/kg SU5416). Control mice were housed in room air and received vehicle (DMSO). Right ventricle pressures (RVP) were recorded with a pressure-conductance transducer. Short axis contiguous 1 mm thick slices were acquired through the heart and great vessels using a Fast Low Angle SHot (FLASH)-cine sequence. Thirteen images were collected throughout each cardiac cycle. RV systolic pressure was elevated in PH and control mice (23.6 ± 5.5 vs. 41.0 ± 11.2 mm Hg, control vs. PH, respectively; p < 0.001, n = 5-11). RV wall thickness was greater in PH mice than control at end-diastole (0.30 ± 0.05, vs. 0.48 + 0.06 mm, control vs. PH, respectively; p < 0.01, n = 6), but measurements were not different at end-systole (control vs. PH, 0.59 ± 0.11 vs. 0.70 + 0.11 mm, respectively). RV ejection fraction was decreased in PH mice (71.9% ± 2.5 vs. 57.6% ± 5.4, control vs. PH, respectively, p < 0.04, n =6). These data demonstrate that MRI is a precise method to longitudinally monitor right ventricle remodeling and cardiac output in mice models of PH.
Original language | English |
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Pages (from-to) | 347–353 |
Number of pages | 6 |
Journal | Experimental Physiology |
Volume | 102 |
Issue number | 3 |
Early online date | 14 Feb 2017 |
DOIs | |
Publication status | Published - 1 Mar 2017 |
Bibliographical note
Acknowledgements: We would like to take this opportunity to dedicate this study to Professor Timothy Bigby who sadly passed away prior to the submission of the manuscript. Professor Bigby was a kind, generous, supportive and inspirational physician and scientist who made major contributions in the field of pulmonary medicine.Grants
The Department of Veterans Affairs, NIH PO1 HL091830, NIH P01 HL66941, NIH HL091061 and the ATS foundation and Pulmonary Hypertension Association supported this study.
Keywords
- cardiac output
- magnetic resonance
- pulmonary hypertension
- hypoxia
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Fiona Murray
- School of Medicine, Medical Sciences & Nutrition, Cardiometabolic Disease
- School of Medicine, Medical Sciences & Nutrition, Aberdeen Cardiovascular and Diabetes Centre
- School of Medicine, Medical Sciences & Nutrition, Medical Sciences - Senior Lecturer
- School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences
Person: Academic