This article aims to envisage future perspectives of the lymphocyte transformation test (LTT). We describe the select innovative techniques, which can be integrated at different stages of the LTT to potentially improve the sensitivity, specificity, or practicability of the LTT. We first focus upon the cell sorting techniques comprising immunomagnetic cell separation and flow cytometry, which can be implemented prior and after the LTT culturing step to concentrate and quantify specific immune cell types. Further, we elaborate upon three important omics techniques such as transcriptomics, proteomics, and metabolomics, which can be integrated downstream of the LTT to analyze molecular changes in specific immune cells following drug induced activation and proliferation. We also develop visions, how state of the art techniques used in other scientific fields, can be transferred and applied in the context of in-vitro detection of drug allergy. [Abstract copyright: Copyright © 2021. Published by Elsevier B.V.]
We thank the European fund for regional development (EFRE), the German Federal State North Rhine-Westphalia (LeitmarktAgentur.NRW), Federal Institute for Drugs and Medical Devices (BfArM), and the Leibniz Institute for Analytical Sciences - ISAS-e.V. for the research project grant. The position of A.F. is financed by the research grant.
The manuscript was written in context with a study related to the improvement of the lymphocyte transformation test which was funded by the European Fund for Regional Development (EFRE) and the German Federal State North Rhine-Westphalia (LeitmarktAgentur.NRW) (funding number: EFRE-0801755) and from own resources of the Federal Institute for Drugs and Medical Devices (BfArM) and the Leibniz Institute for Analytical Sciences - ISAS-e.V., Dortmund, Germany.
- Cell sorting by immunomagnetic cell separation and flow cytometry
- Drug allergy
- Future perspectives in vitro diagnostics
- Lymphocyte transformation test (LTT)
- Omics by transcriptomics proteomics and metabolomics
- T cell activation and proliferation