Candida albicans, a commensal yeast that is also responsible for frequent superficial and disseminated infections in humans. We have generated an ORFeome collection composed of 5,099 ORFs cloned in a Gateway™ donor vector, representing 83% of the currently annotated coding sequences of C. albicans. Sequencing data of the cloned ORFs are available in the CandidaOrfDB database at http://candidaorfeome.eu. We also engineered 49 expression vectors with a choice of promoters, tags, and selection markers and demonstrated their applicability to the study of target ORFs transferred from the
C. albicans ORFeome. In addition, the use of the ORFeome in the detection of protein-protein interaction was demonstrated. Mating-compatible strains as well as Gateway™-compatible two-hybrid vectors were engineered, validated and used in a proof of concept experiment. These unique and valuable resources should greatly facilitate future functional studies in C. albicans and the elucidation of mechanisms that underlie its pathogenicity.
Bibliographical noteThis work has been supported by a Biomedical Resources grant from the Wellcome Trust (The Candida albicans ORFeome project, 088858/Z/09/Z to CAM and CD), the European Commission (FINSysB, PITN-GA-2008-214004 to CD), the Agence Nationale de la Recherche (KANJI, ANR-08-MIE-033-01 to CD; CANDICOL, ANR-10-01 to CD) and the Interuniversity Attraction Poles Programme initiated by the Belgian Science Policy Office for funding (IAP P7/28 to PVD). CAM would like to acknowledge support from a Medical Research Council New Investigator Award (G0400284) and the MRC Centre for Medical Mycology and the University of Aberdeen (MR/M026663/1). VC was the recipient of a PhD fellowship in the framework of the FINSysB consortium (PITN-A-2008-214004). AN was the recipient of a PhD fellowship from the DIM-MalInf Région Ile-de-France. EP was the recipient of a post-doctoral fellowship in the framework of the C. albicans ORFeome project (WT088858MA). TR was the recipient of a postdoctoral fellowship in the framework of the NPARI consortium (LSHE-CT-2006-037692). UZ was the recipient of a postdoctoral fellowship in the framework of Programme Fungi from Institut Carnot-Pasteur Maladies Infectieuses. SZ was the recipient from post-doctoral fellowships in the framework of the FINSysB (PITN-GA-2008-214004) and KANJI (ANR-08-MIE-033-01) consortia. HT was the recipient of a KU Leuven CREA grant (2011-2013). High throughput sequencing has been performed on the Genomics Platform, member of France Génomique consortium (ANR10-INBS-09-08). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We acknowledge support from the French Government’s Investissement d’Avenir program (Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases, ANR-10-LABX-62-IBEID).
Erratum: Generating genomic platforms to study Candida albicans pathogenesis
Mélanie Legrand, Sophie Bachellier-Bassi, Keunsook K. Lee, Yogesh Chaudhari, Hélène Tournu, Laurence Arbogast, Hélène Boyer, Murielle Chauvel, Vitor Cabral, Corinne Maufrais, Audrey Nesseir, Irena Maslanka, Emmanuelle Permal, Tristan Rossignol, Louise A. Walker, Ute Zeidler, Sadri Znaidi, Floris Schoeters, Charlotte Majgier, Renaud A. Julien, 2018, vol. 46, issue 16, p. 8664. Nucleic acids research http://dx.doi.org/10.1093/nar/gky747
- Candida albicans
- Two-hybrid system
- Gateway recombination cloning system