Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

Carylyn J Marek; Gary A Cameron; Lucy J Elrick; Gabrielle M Hawksworth; Matthew C Wright

doi:10.1042/BJ20021545

Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

Carylyn J Marek, Gary A Cameron, Lucy J Elrick, Gabrielle M Hawksworth, Matthew C Wright

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

Original language	English
Pages (from-to)	763-769
Number of pages	7
Journal	Biochemical Journal
Volume	370
Issue number	3
DOIs	https://doi.org/10.1042/BJ20021545
Publication status	Published - 15 Mar 2003

Keywords

Animals
Cell Differentiation
Cell Line
Cytochrome P-450 Enzyme System
Dexamethasone
Glucocorticoids
Hepatocytes
Male
Pancreas
Phenotype
Rats
Rats, Sprague-Dawley
Testosterone

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10.1042/BJ20021545

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title = "Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro",

abstract = "The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.",

keywords = "Animals, Cell Differentiation, Cell Line, Cytochrome P-450 Enzyme System, Dexamethasone, Glucocorticoids, Hepatocytes, Male, Pancreas, Phenotype, Rats, Rats, Sprague-Dawley, Testosterone",

author = "Marek, {Carylyn J} and Cameron, {Gary A} and Elrick, {Lucy J} and Hawksworth, {Gabrielle M} and Wright, {Matthew C}",

year = "2003",

month = mar,

day = "15",

doi = "10.1042/BJ20021545",

language = "English",

volume = "370",

pages = "763--769",

journal = "Biochemical Journal",

issn = "0264-6021",

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TY - JOUR

T1 - Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

AU - Marek, Carylyn J

AU - Cameron, Gary A

AU - Elrick, Lucy J

AU - Hawksworth, Gabrielle M

AU - Wright, Matthew C

PY - 2003/3/15

Y1 - 2003/3/15

N2 - The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

AB - The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

KW - Animals

KW - Cell Differentiation

KW - Cell Line

KW - Cytochrome P-450 Enzyme System

KW - Dexamethasone

KW - Glucocorticoids

KW - Hepatocytes

KW - Male

KW - Pancreas

KW - Phenotype

KW - Rats

KW - Rats, Sprague-Dawley

KW - Testosterone

U2 - 10.1042/BJ20021545

DO - 10.1042/BJ20021545

M3 - Article

C2 - 12542397

SN - 0264-6021

VL - 370

SP - 763

EP - 769

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

Abstract

Keywords

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